A search for differentiation markers in prostatic fluid components with two‐dimensional electrophoresis and multiple protein detectors

A. H. Johnson*, D. J. Merchant, J. Norman, N. Bourgeois, C. Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Components of prostatic fluid resolved by two‐dimensional polyacrylamide gel electrophoresis were detected by dye, silver stain, and autoradiography. Five components identified by autoradiography were not recognized by the dye or silver stain. While labeled components were found in primary fractions of split ejaculates and seminal fluid in addition to prostatic fluid, they have not been found in tissue extracts of heterologous organs or human serum.

Original languageEnglish (US)
Pages (from-to)429-439
Number of pages11
JournalThe Prostate
Volume7
Issue number4
DOIs
StatePublished - 1985

Keywords

  • 2‐DEP
  • Coomassie
  • Iso‐Dalt electrophoresis
  • autoradiography
  • prostatic fluid
  • silver stain

ASJC Scopus subject areas

  • Oncology
  • Urology

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