A secretable serine proteinase with highly restricted specificity from cytolytic T lymphocytes inactivates retrovirus-associated reverse transcriptase

Hans Georg Simon; Ulli Fruth; Michael D. Kramer; Markus M. Simon

doi:10.1016/0014-5793(87)80318-6

A secretable serine proteinase with highly restricted specificity from cytolytic T lymphocytes inactivates retrovirus-associated reverse transcriptase

Hans Georg Simon, Ulli Fruth, Michael D. Kramer, Markus M. Simon^*

^*Corresponding author for this work

Pediatrics

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26 Scopus citations

Abstract

TSP-1, a murine T cell specific proteinase, is expressed in cytolytic T lymphocytes and secreted upon their interaction with antigen bearing target cells. In searching for possible extracellular substrates of the enzyme in the physiological environment of cytolytic effector cells, we have investigated the proteolytic activity of TSP-1 on retroviral proteins. It is shown that reverse transcriptase derived from the retrovirus Moloney murine leukemia virus is inactivated by TSP-1 via limited proteolysis. The data suggest the possibility that cytolytic T lymphocytes are able to interfere with retroviral replication by secreting a serine proteinase which degrades viral proteins.

Original language	English (US)
Pages (from-to)	352-360
Number of pages	9
Journal	FEBS Letters
Volume	223
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(87)80318-6
State	Published - Nov 2 1987

Keywords

(Cytolytic T cell)
Reverse transcriptase
Serine proteinase
Virus replication

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(87)80318-6

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title = "A secretable serine proteinase with highly restricted specificity from cytolytic T lymphocytes inactivates retrovirus-associated reverse transcriptase",

abstract = "TSP-1, a murine T cell specific proteinase, is expressed in cytolytic T lymphocytes and secreted upon their interaction with antigen bearing target cells. In searching for possible extracellular substrates of the enzyme in the physiological environment of cytolytic effector cells, we have investigated the proteolytic activity of TSP-1 on retroviral proteins. It is shown that reverse transcriptase derived from the retrovirus Moloney murine leukemia virus is inactivated by TSP-1 via limited proteolysis. The data suggest the possibility that cytolytic T lymphocytes are able to interfere with retroviral replication by secreting a serine proteinase which degrades viral proteins.",

keywords = "(Cytolytic T cell), Reverse transcriptase, Serine proteinase, Virus replication",

author = "Simon, {Hans Georg} and Ulli Fruth and Kramer, {Michael D.} and Simon, {Markus M.}",

note = "Funding Information: We thank MS G. Nerz and M. Prester for ex-cellentt echnicala ssistanceT. he authorsa lso thank Drs H. Eibel, K. Eichmann, G. KGhler, J. Langhorne, R. Lamers, G. McMaster, P. Nielson, and H.D. Wolf for critically reading this manuscript,D rs H. Eibel and R. Wallich for their help in screeningp rotein sequenced atab anks,and MS R. Brugger and R. Schneiderf or secretarial help. This work was in part supportedb y a grant from the Deutsche Forschungsgemeinschaft Si 214/j-4.",

year = "1987",

month = nov,

day = "2",

doi = "10.1016/0014-5793(87)80318-6",

language = "English (US)",

volume = "223",

pages = "352--360",

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T1 - A secretable serine proteinase with highly restricted specificity from cytolytic T lymphocytes inactivates retrovirus-associated reverse transcriptase

AU - Simon, Hans Georg

AU - Fruth, Ulli

AU - Kramer, Michael D.

AU - Simon, Markus M.

N1 - Funding Information: We thank MS G. Nerz and M. Prester for ex-cellentt echnicala ssistanceT. he authorsa lso thank Drs H. Eibel, K. Eichmann, G. KGhler, J. Langhorne, R. Lamers, G. McMaster, P. Nielson, and H.D. Wolf for critically reading this manuscript,D rs H. Eibel and R. Wallich for their help in screeningp rotein sequenced atab anks,and MS R. Brugger and R. Schneiderf or secretarial help. This work was in part supportedb y a grant from the Deutsche Forschungsgemeinschaft Si 214/j-4.

PY - 1987/11/2

Y1 - 1987/11/2

N2 - TSP-1, a murine T cell specific proteinase, is expressed in cytolytic T lymphocytes and secreted upon their interaction with antigen bearing target cells. In searching for possible extracellular substrates of the enzyme in the physiological environment of cytolytic effector cells, we have investigated the proteolytic activity of TSP-1 on retroviral proteins. It is shown that reverse transcriptase derived from the retrovirus Moloney murine leukemia virus is inactivated by TSP-1 via limited proteolysis. The data suggest the possibility that cytolytic T lymphocytes are able to interfere with retroviral replication by secreting a serine proteinase which degrades viral proteins.

AB - TSP-1, a murine T cell specific proteinase, is expressed in cytolytic T lymphocytes and secreted upon their interaction with antigen bearing target cells. In searching for possible extracellular substrates of the enzyme in the physiological environment of cytolytic effector cells, we have investigated the proteolytic activity of TSP-1 on retroviral proteins. It is shown that reverse transcriptase derived from the retrovirus Moloney murine leukemia virus is inactivated by TSP-1 via limited proteolysis. The data suggest the possibility that cytolytic T lymphocytes are able to interfere with retroviral replication by secreting a serine proteinase which degrades viral proteins.

KW - (Cytolytic T cell)

KW - Reverse transcriptase

KW - Serine proteinase

KW - Virus replication

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AN - SCOPUS:0023642598

VL - 223

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EP - 360

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -

A secretable serine proteinase with highly restricted specificity from cytolytic T lymphocytes inactivates retrovirus-associated reverse transcriptase

Abstract

Keywords

ASJC Scopus subject areas

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