Abstract
TSP-1, a murine T cell specific proteinase, is expressed in cytolytic T lymphocytes and secreted upon their interaction with antigen bearing target cells. In searching for possible extracellular substrates of the enzyme in the physiological environment of cytolytic effector cells, we have investigated the proteolytic activity of TSP-1 on retroviral proteins. It is shown that reverse transcriptase derived from the retrovirus Moloney murine leukemia virus is inactivated by TSP-1 via limited proteolysis. The data suggest the possibility that cytolytic T lymphocytes are able to interfere with retroviral replication by secreting a serine proteinase which degrades viral proteins.
Original language | English (US) |
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Pages (from-to) | 352-360 |
Number of pages | 9 |
Journal | FEBS Letters |
Volume | 223 |
Issue number | 2 |
DOIs | |
State | Published - Nov 2 1987 |
Funding
We thank MS G. Nerz and M. Prester for ex-cellentt echnicala ssistanceT. he authorsa lso thank Drs H. Eibel, K. Eichmann, G. KGhler, J. Langhorne, R. Lamers, G. McMaster, P. Nielson, and H.D. Wolf for critically reading this manuscript,D rs H. Eibel and R. Wallich for their help in screeningp rotein sequenced atab anks,and MS R. Brugger and R. Schneiderf or secretarial help. This work was in part supportedb y a grant from the Deutsche Forschungsgemeinschaft Si 214/j-4.
Keywords
- (Cytolytic T cell)
- Reverse transcriptase
- Serine proteinase
- Virus replication
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Biophysics
- Structural Biology
- Biochemistry
- Cell Biology