A selective chemical probe for coenzyme A-requiring enzymes

Yousang Hwang; Paul R. Thompson; Ling Wang; Lihua Jiang; Neil L. Kelleher; Philip A. Cole

doi:10.1002/anie.200702485

A selective chemical probe for coenzyme A-requiring enzymes

Yousang Hwang^*, Paul R. Thompson, Ling Wang, Lihua Jiang, Neil L. Kelleher, Philip A. Cole

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

(Chemical Equation Presented) Tagging transferases: A coenzyme A (CoA)-based affinity probe with a sulfoxycarbamate functionality can selectively identify several acetyltransferases relative to other enzymes and proteins. It leaves behind a desthiobiotin tag that can be used for western blotting and mass spectrometric characterization (see picture; Nu = nucleophile).

Original language	English (US)
Pages (from-to)	7621-7624
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	40
DOIs	https://doi.org/10.1002/anie.200702485
State	Published - 2007

Keywords

Cofactors
Enzymes
Protein modifications
Proteomics
Transferases

ASJC Scopus subject areas

General Chemistry
Catalysis

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10.1002/anie.200702485

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abstract = "(Chemical Equation Presented) Tagging transferases: A coenzyme A (CoA)-based affinity probe with a sulfoxycarbamate functionality can selectively identify several acetyltransferases relative to other enzymes and proteins. It leaves behind a desthiobiotin tag that can be used for western blotting and mass spectrometric characterization (see picture; Nu = nucleophile).",

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year = "2007",

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T1 - A selective chemical probe for coenzyme A-requiring enzymes

AU - Hwang, Yousang

AU - Thompson, Paul R.

AU - Wang, Ling

AU - Jiang, Lihua

AU - Kelleher, Neil L.

AU - Cole, Philip A.

PY - 2007

Y1 - 2007

N2 - (Chemical Equation Presented) Tagging transferases: A coenzyme A (CoA)-based affinity probe with a sulfoxycarbamate functionality can selectively identify several acetyltransferases relative to other enzymes and proteins. It leaves behind a desthiobiotin tag that can be used for western blotting and mass spectrometric characterization (see picture; Nu = nucleophile).

AB - (Chemical Equation Presented) Tagging transferases: A coenzyme A (CoA)-based affinity probe with a sulfoxycarbamate functionality can selectively identify several acetyltransferases relative to other enzymes and proteins. It leaves behind a desthiobiotin tag that can be used for western blotting and mass spectrometric characterization (see picture; Nu = nucleophile).

KW - Cofactors

KW - Enzymes

KW - Protein modifications

KW - Proteomics

KW - Transferases

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U2 - 10.1002/anie.200702485

DO - 10.1002/anie.200702485

M3 - Article

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AN - SCOPUS:35048849835

SN - 1433-7851

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 40

ER -

A selective chemical probe for coenzyme A-requiring enzymes

Abstract

Keywords

ASJC Scopus subject areas

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