A selective chemical probe for coenzyme A-requiring enzymes

Yousang Hwang*, Paul R. Thompson, Ling Wang, Lihua Jiang, Neil L. Kelleher, Philip A. Cole

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

(Chemical Equation Presented) Tagging transferases: A coenzyme A (CoA)-based affinity probe with a sulfoxycarbamate functionality can selectively identify several acetyltransferases relative to other enzymes and proteins. It leaves behind a desthiobiotin tag that can be used for western blotting and mass spectrometric characterization (see picture; Nu = nucleophile).

Original languageEnglish (US)
Pages (from-to)7621-7624
Number of pages4
JournalAngewandte Chemie - International Edition
Volume46
Issue number40
DOIs
StatePublished - Oct 16 2007

Keywords

  • Cofactors
  • Enzymes
  • Protein modifications
  • Proteomics
  • Transferases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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