A sensitive assay of cytotoxicity applicable to mixed cell populations

Reggie E. Duerst*, Christopher N. Frantz

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The fluorescent vital dye, Hoechst 33342, was used to stain cultured cells prior to assay of antibody dependent complement mediated cytotoxicity. The fluorescence of nonviable dye stained cells is quenched by cellular uptake of trypan blue, but trypan blue excluding cells remain intensely fluorescent. Detection by fluorescence microscopy of one viable prestained cell per 105 unstained cells was accurate and reliable. The technique was found to have sensitivity equal to a clonogenic assay for measuring cytotoxicity. The dye stained cell assay may be used to measure depletion of a selected cell type, when those cells are stained prior to mixing with another cell population. This technique may prove useful to study model systems for depletion of tumor cells or T-cells from bone marrow.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalJournal of Immunological Methods
Volume82
Issue number1
DOIs
StatePublished - Sep 3 1985

Keywords

  • Hoechst 33342
  • clonogenic assay
  • complement mediated cytotoxicity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A sensitive assay of cytotoxicity applicable to mixed cell populations'. Together they form a unique fingerprint.

Cite this