A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

A. R. Gibson; D. I. Hansma; J. C. Houk; F. R. Robinson

doi:10.1016/0006-8993(84)91423-9

A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

A. R. Gibson^*, D. I. Hansma, J. C. Houk, F. R. Robinson

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

270 Scopus citations

Abstract

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

Original language	English (US)
Pages (from-to)	235-241
Number of pages	7
Journal	Brain research
Volume	298
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(84)91423-9
State	Published - Apr 30 1984

Keywords

TMB
WGA-HRP
lectin
wheat germ agglutinin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(84)91423-9

Cite this

@article{4089ee6fc01341eb8f71dc8defe8efec,

title = "A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS",

abstract = "A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.",

keywords = "TMB, WGA-HRP, lectin, wheat germ agglutinin",

author = "Gibson, {A. R.} and Hansma, {D. I.} and Houk, {J. C.} and Robinson, {F. R.}",

year = "1984",

month = apr,

day = "30",

doi = "10.1016/0006-8993(84)91423-9",

language = "English (US)",

volume = "298",

pages = "235--241",

journal = "Brain research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

AU - Gibson, A. R.

AU - Hansma, D. I.

AU - Houk, J. C.

AU - Robinson, F. R.

PY - 1984/4/30

Y1 - 1984/4/30

N2 - A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

AB - A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

KW - TMB

KW - WGA-HRP

KW - lectin

KW - wheat germ agglutinin

UR - http://www.scopus.com/inward/record.url?scp=0021183477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021183477&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(84)91423-9

DO - 10.1016/0006-8993(84)91423-9

M3 - Article

C2 - 6202368

AN - SCOPUS:0021183477

SN - 0006-8993

VL - 298

SP - 235

EP - 241

JO - Brain research

JF - Brain research

IS - 2

ER -

A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this