A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

A. R. Gibson*, D. I. Hansma, J. C. Houk, F. R. Robinson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

264 Scopus citations

Abstract

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalBrain research
Volume298
Issue number2
DOIs
StatePublished - Apr 30 1984

Keywords

  • TMB
  • WGA-HRP
  • lectin
  • wheat germ agglutinin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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