A signature of four circulating micrornas as potential biomarkers for diagnosing early-stage breast cancer

Maha M. Itani; Farah J. Nassar; Arafat H. Tfayli; Rabih S. Talhouk; Ghada K. Chamandi; Abdul Rahman S. Itani; Joelle Makoukji; Rose Mary N. Boustany; Lifang Hou; Nathalie K. Zgheib; Rihab R. Nasr

doi:10.3390/ijms22116121

A signature of four circulating micrornas as potential biomarkers for diagnosing early-stage breast cancer

Maha M. Itani, Farah J. Nassar, Arafat H. Tfayli, Rabih S. Talhouk, Ghada K. Chamandi, Abdul Rahman S. Itani, Joelle Makoukji, Rose Mary N. Boustany, Lifang Hou, Nathalie K. Zgheib^*, Rihab R. Nasr^*

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Breast cancer (BC) is the most predominant type of cancer among women. The aim of this study is to find new biomarkers that can help in early detection of BC, especially for those who are too young to be screened using mammography as per guidelines. Using microRNA microarray, we previously showed dysregulation of 74 microRNAs in tumors from early BC patients as compared with normal adjacent tissues, which we were interested in studying in blood circulation. In this study, we investigated the expression of 12 microRNA (miR-21/miR-155/miR-23a/miR-130a/miR-145/miR-425-5p/miR-139-5p/miR-451/miR-195/miR-125b/miR-100, and miR-182) in the plasma of 41 newly diagnosed Lebanese BC patients with early invasive ductal carcinoma as compared with 32 healthy controls. Total RNA was extracted from plasma, and expression levels of miRNA of interest were measured using RT-qPCR followed by statistical analysis; miR-21, miR-155, miR-23a, miR-130a, miR-145, miR-425-5p, and miR-139-5p were significantly upregulated and miR-451 was significantly downregulated, in the plasma of BC patients as compared with healthy controls. The positively correlated miR-23a, miR-21, and miR-130a had a high diagnostic accuracy (86%). Importantly, the combination of miR-145/miR-425-5p/miR-139-5p/miR-130a scored the highest diagnostic accuracy of 95% with AUC = 0.97 (sensitivity 97% and specificity 91%). MicroRNAs are promising non-invasive diagnostic biomarkers for early-stage BC with the panel of miR-145/miR-425-5p/miR-139-5p/miR-130a having the highest diagnostic accuracy.

Original language	English (US)
Article number	6121
Journal	International journal of molecular sciences
Volume	22
Issue number	11
DOIs	https://doi.org/10.3390/ijms22116121
State	Published - Jun 1 2021

Funding

Funding: We would like to thank the Medical Practice Plan (MPP), the Lebanese National Council for Scientific Research (CNRS-L), and the Northwestern University Kiphart award for funding the project.

Keywords

Breast cancer
Circulating biomarkers
Diagnosis
Early stage
Liquid biopsy
MicroRNA
ROC curve

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijms22116121

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Itani, M. M., Nassar, F. J., Tfayli, A. H., Talhouk, R. S., Chamandi, G. K., Itani, A. R. S., Makoukji, J., Boustany, R. M. N., Hou, L., Zgheib, N. K., & Nasr, R. R. (2021). A signature of four circulating micrornas as potential biomarkers for diagnosing early-stage breast cancer. International journal of molecular sciences, 22(11), Article 6121. https://doi.org/10.3390/ijms22116121

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abstract = "Breast cancer (BC) is the most predominant type of cancer among women. The aim of this study is to find new biomarkers that can help in early detection of BC, especially for those who are too young to be screened using mammography as per guidelines. Using microRNA microarray, we previously showed dysregulation of 74 microRNAs in tumors from early BC patients as compared with normal adjacent tissues, which we were interested in studying in blood circulation. In this study, we investigated the expression of 12 microRNA (miR-21/miR-155/miR-23a/miR-130a/miR-145/miR-425-5p/miR-139-5p/miR-451/miR-195/miR-125b/miR-100, and miR-182) in the plasma of 41 newly diagnosed Lebanese BC patients with early invasive ductal carcinoma as compared with 32 healthy controls. Total RNA was extracted from plasma, and expression levels of miRNA of interest were measured using RT-qPCR followed by statistical analysis; miR-21, miR-155, miR-23a, miR-130a, miR-145, miR-425-5p, and miR-139-5p were significantly upregulated and miR-451 was significantly downregulated, in the plasma of BC patients as compared with healthy controls. The positively correlated miR-23a, miR-21, and miR-130a had a high diagnostic accuracy (86%). Importantly, the combination of miR-145/miR-425-5p/miR-139-5p/miR-130a scored the highest diagnostic accuracy of 95% with AUC = 0.97 (sensitivity 97% and specificity 91%). MicroRNAs are promising non-invasive diagnostic biomarkers for early-stage BC with the panel of miR-145/miR-425-5p/miR-139-5p/miR-130a having the highest diagnostic accuracy.",

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AU - Itani, Maha M.

AU - Nassar, Farah J.

AU - Tfayli, Arafat H.

AU - Talhouk, Rabih S.

AU - Chamandi, Ghada K.

AU - Itani, Abdul Rahman S.

AU - Makoukji, Joelle

AU - Boustany, Rose Mary N.

AU - Hou, Lifang

AU - Zgheib, Nathalie K.

AU - Nasr, Rihab R.

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AB - Breast cancer (BC) is the most predominant type of cancer among women. The aim of this study is to find new biomarkers that can help in early detection of BC, especially for those who are too young to be screened using mammography as per guidelines. Using microRNA microarray, we previously showed dysregulation of 74 microRNAs in tumors from early BC patients as compared with normal adjacent tissues, which we were interested in studying in blood circulation. In this study, we investigated the expression of 12 microRNA (miR-21/miR-155/miR-23a/miR-130a/miR-145/miR-425-5p/miR-139-5p/miR-451/miR-195/miR-125b/miR-100, and miR-182) in the plasma of 41 newly diagnosed Lebanese BC patients with early invasive ductal carcinoma as compared with 32 healthy controls. Total RNA was extracted from plasma, and expression levels of miRNA of interest were measured using RT-qPCR followed by statistical analysis; miR-21, miR-155, miR-23a, miR-130a, miR-145, miR-425-5p, and miR-139-5p were significantly upregulated and miR-451 was significantly downregulated, in the plasma of BC patients as compared with healthy controls. The positively correlated miR-23a, miR-21, and miR-130a had a high diagnostic accuracy (86%). Importantly, the combination of miR-145/miR-425-5p/miR-139-5p/miR-130a scored the highest diagnostic accuracy of 95% with AUC = 0.97 (sensitivity 97% and specificity 91%). MicroRNAs are promising non-invasive diagnostic biomarkers for early-stage BC with the panel of miR-145/miR-425-5p/miR-139-5p/miR-130a having the highest diagnostic accuracy.

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KW - ROC curve

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A signature of four circulating micrornas as potential biomarkers for diagnosing early-stage breast cancer

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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