A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs

Matthew A. Butzler, Jennifer L. Reed, Sally M McFall*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.

Original languageEnglish (US)
Pages (from-to)182-184
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume89
Issue number3
DOIs
StatePublished - Nov 1 2017

Fingerprint

Chlamydia trachomatis
Routine Diagnostic Tests
Nucleic Acids
Sensitivity and Specificity
Polymerase Chain Reaction
DNA

Keywords

  • Chlamydia trachomatis
  • Diagnosis
  • Quantitative PCR
  • Sample prep
  • Sexually transmitted disease

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100{\%} sensitivity and specificity.",
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author = "Butzler, {Matthew A.} and Reed, {Jennifer L.} and McFall, {Sally M}",
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A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs. / Butzler, Matthew A.; Reed, Jennifer L.; McFall, Sally M.

In: Diagnostic Microbiology and Infectious Disease, Vol. 89, No. 3, 01.11.2017, p. 182-184.

Research output: Contribution to journalArticle

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AU - Reed, Jennifer L.

AU - McFall, Sally M

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KW - Diagnosis

KW - Quantitative PCR

KW - Sample prep

KW - Sexually transmitted disease

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