@article{b996a8393f8a474fad6ae0205f3815c4,
title = "A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs",
abstract = "A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.",
keywords = "Chlamydia trachomatis, Diagnosis, Quantitative PCR, Sample prep, Sexually transmitted disease",
author = "Butzler, {Matthew A.} and Reed, {Jennifer L.} and McFall, {Sally M.}",
note = "Funding Information: We thank Johns Hopkins Center for STD Point-of-Care Tests for providing the Chlamydia trachomatis swab specimens and microbial DNA for the specificity panel, and special thanks to Justin Hardick for providing us with quantified CT DNA to standardize the CT-infected McCoy cell preps. MAB and SMM were supported by SP0030079 and SP0035724 JHU POCT, Johns Hopkins Center for STD Point-of-Care Tests. The sponsor had no role in study design or collection, analysis or interpretation of data presented in this report. Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
month = nov,
doi = "10.1016/j.diagmicrobio.2017.08.007",
language = "English (US)",
volume = "89",
pages = "182--184",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "3",
}
