A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Frank Eckerdt*, Angel Alvarez, Jonathan Bell, Constadina Arvanitis, Asneha Iqbal, Ahmet D. Arslan, Bo Hu, Shi Yuan Cheng, Stewart Goldman, Leonidas C. Platanias

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)–based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

Original languageEnglish (US)
Pages (from-to)43-46
Number of pages4
JournalBioTechniques
Volume60
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Tumors
Neoplastic Stem Cells
Throughput
Staining and Labeling
Stem cells
Costs and Cost Analysis
Screening
Costs
Neoplasms
Acridine Orange
Preclinical Drug Evaluations
Fluorescence microscopy
Fluorescence Microscopy
Cell culture
Pharmaceutical Preparations
Assays
Cell Survival
Cells
Growth

Keywords

  • Acridine orange
  • Cancer stem cell
  • Glioblastoma
  • Microscopy
  • Neurospheres
  • Tumor spheroids

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)–based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.",
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A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids. / Eckerdt, Frank; Alvarez, Angel; Bell, Jonathan; Arvanitis, Constadina; Iqbal, Asneha; Arslan, Ahmet D.; Hu, Bo; Cheng, Shi Yuan; Goldman, Stewart; Platanias, Leonidas C.

In: BioTechniques, Vol. 60, No. 1, 01.01.2016, p. 43-46.

Research output: Contribution to journalArticle

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AU - Eckerdt, Frank

AU - Alvarez, Angel

AU - Bell, Jonathan

AU - Arvanitis, Constadina

AU - Iqbal, Asneha

AU - Arslan, Ahmet D.

AU - Hu, Bo

AU - Cheng, Shi Yuan

AU - Goldman, Stewart

AU - Platanias, Leonidas C.

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