A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Frank Eckerdt; Angel Alvarez; Jonathan Bell; Constadina Arvanitis; Asneha Iqbal; Ahmet D. Arslan; Bo Hu; Shi Yuan Cheng; Stewart Goldman; Leonidas C. Platanias

doi:10.2144/000114372

A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Frank Eckerdt^*, Angel Alvarez, Jonathan Bell, Constadina Arvanitis, Asneha Iqbal, Ahmet D. Arslan, Bo Hu, Shi Yuan Cheng, Stewart Goldman, Leonidas C. Platanias

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)–based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

Original language	English (US)
Pages (from-to)	43-46
Number of pages	4
Journal	BioTechniques
Volume	60
Issue number	1
DOIs	https://doi.org/10.2144/000114372
State	Published - Jan 2016

Keywords

Acridine orange
Cancer stem cell
Glioblastoma
Microscopy
Neurospheres
Tumor spheroids

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2144/000114372

Cite this

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title = "A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids",

abstract = "Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)–based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.",

keywords = "Acridine orange, Cancer stem cell, Glioblastoma, Microscopy, Neurospheres, Tumor spheroids",

author = "Frank Eckerdt and Angel Alvarez and Jonathan Bell and Constadina Arvanitis and Asneha Iqbal and Arslan, \{Ahmet D.\} and Bo Hu and Cheng, \{Shi Yuan\} and Stewart Goldman and Platanias, \{Leonidas C.\}",

year = "2016",

month = jan,

doi = "10.2144/000114372",

language = "English (US)",

volume = "60",

pages = "43--46",

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AU - Eckerdt, Frank

AU - Alvarez, Angel

AU - Bell, Jonathan

AU - Arvanitis, Constadina

AU - Iqbal, Asneha

AU - Arslan, Ahmet D.

AU - Hu, Bo

AU - Cheng, Shi Yuan

AU - Goldman, Stewart

AU - Platanias, Leonidas C.

PY - 2016/1

Y1 - 2016/1

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AB - Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)–based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

KW - Acridine orange

KW - Cancer stem cell

KW - Glioblastoma

KW - Microscopy

KW - Neurospheres

KW - Tumor spheroids

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A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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