A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases

Catherine McKeon; B. Wolf

doi:10.1007/BF01945114

A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases

Catherine McKeon^*, B. Wolf

^*Corresponding author for this work

Pediatrics

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Abstract

Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purifiy the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

Original language	English (US)
Pages (from-to)	281-282
Number of pages	2
Journal	Experientia
Volume	38
Issue number	2
DOIs	https://doi.org/10.1007/BF01945114
State	Published - Feb 1 1982

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01945114

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abstract = "Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purifiy the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.",

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AB - Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purifiy the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

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A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases

Abstract

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