A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases

Catherine McKeon*, B. Wolf

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purifiy the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

Original languageEnglish (US)
Pages (from-to)281-282
Number of pages2
JournalExperientia
Volume38
Issue number2
DOIs
StatePublished - Feb 1 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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