A simple method has been described for the preparation of small amounts of highly purified factor VIII (antihemophilic factor). Cryoprecipitate is clotted with a purified extract of snake venom (Arvin), and the resultant product is filtered through Sepharose. The mean specific activity of factor VIII prepared by this method is 124 U. per milligram of protein, representing an 8,665-fold purification of the factor.
|Original language||English (US)|
|Number of pages||6|
|Journal||The Journal of Laboratory and Clinical Medicine|
|State||Published - Jan 1 1971|
ASJC Scopus subject areas
- Pathology and Forensic Medicine