TY - JOUR
T1 - A simple quantitative assay for studying the invasive potential of high and low human metastatic variants
AU - Hendrix, Mary J.C.
AU - Seftor, Elisabeth A.
AU - Seftor, Richard E.B.
AU - Fidler, Isaiah J.
N1 - Funding Information:
The authors would like to gratefully acknowledge the helpful suggestions of Dr. Danny R. Welch (Cancer and Viral Diseases Reseach, The Upjohn Company), Dr. Ronald L. Misiorowski and Mr. Cisco Tellez (Department of Surgery, University of Arizona, College of Medicine), and Dr. Frank L. Meyskens, Jr. (Arizona Cancer Center). We are especially thankful to Drs. George R. Martin and Hynda K. Kleinman of NIDR/NIH for the Matrigel samples. This research was supported by NIH/NCI lROlCA4247!5-02 to MJCH and NIH Training Grant CA09213 and ACS Institutional Grant 964812 to REBS.
PY - 1987/12
Y1 - 1987/12
N2 - This paper presents a more reliable model for studying the extent of tumor cell migration and invasion in vitro. Polycarbonate filters were uniformly coated with a reconstituted basement membrane material and allowed to dry; each filter measured 0.035 mm in thickness when hydrated with media. Subsequently, the membrane-coated filters were suspended in Membrane Invasion Culture System (MICS) chambers, and high (A375M) and low (A375P) metastatic variants of human melanoma cells were seeded onto the membranes and allowed to incubate for 3 days. At the end of this period, cells were examined morphologically, and the invasive cells of both metastatic variants were collected, stained and counted microscopically. The tumor cells could be seen attached to the reconstituted basement membrane, buried within it, and forming colony-like aggregates in the matrix. It was determined that approximately twice as many A375M cells invaded the artificial biological matrix compared with the A375P cells (P < 0.0005). Substantially more cells from each variant invaded uncoated polycarbonate filters, thus indicating the selective barrier imposed by the Matrigel. The utilization of such a reconstituted matrix for in vitro invasion studies allows one the opportunity to examine tumor cell attachment, migration and invasion of a uniform matrix over an extended period of time.
AB - This paper presents a more reliable model for studying the extent of tumor cell migration and invasion in vitro. Polycarbonate filters were uniformly coated with a reconstituted basement membrane material and allowed to dry; each filter measured 0.035 mm in thickness when hydrated with media. Subsequently, the membrane-coated filters were suspended in Membrane Invasion Culture System (MICS) chambers, and high (A375M) and low (A375P) metastatic variants of human melanoma cells were seeded onto the membranes and allowed to incubate for 3 days. At the end of this period, cells were examined morphologically, and the invasive cells of both metastatic variants were collected, stained and counted microscopically. The tumor cells could be seen attached to the reconstituted basement membrane, buried within it, and forming colony-like aggregates in the matrix. It was determined that approximately twice as many A375M cells invaded the artificial biological matrix compared with the A375P cells (P < 0.0005). Substantially more cells from each variant invaded uncoated polycarbonate filters, thus indicating the selective barrier imposed by the Matrigel. The utilization of such a reconstituted matrix for in vitro invasion studies allows one the opportunity to examine tumor cell attachment, migration and invasion of a uniform matrix over an extended period of time.
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U2 - 10.1016/0304-3835(87)90209-6
DO - 10.1016/0304-3835(87)90209-6
M3 - Article
C2 - 3690504
AN - SCOPUS:0023499529
SN - 0304-3835
VL - 38
SP - 137
EP - 147
JO - Cancer Letters
JF - Cancer Letters
IS - 1-2
ER -