1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10-13 moles/ml of ATP can be repeatably and reliably detected.
|Original language||English (US)|
|Number of pages||11|
|Journal||Comparative Biochemistry and Physiology -- Part A: Physiology|
|State||Published - Jul 1 1974|
- chloride inhibition
- firefly lantern extract
ASJC Scopus subject areas