A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues

Eugene M. Silinsky

doi:10.1016/0300-9629(74)90739-7

A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues

Eugene M. Silinsky^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10^-13 moles/ml of ATP can be repeatably and reliably detected.

Original language	English (US)
Pages (from-to)	561-571
Number of pages	11
Journal	Comparative Biochemistry and Physiology -- Part A: Physiology
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/0300-9629(74)90739-7
State	Published - Jul 1 1974

Keywords

ADP
ATP
chloride inhibition
firefly lantern extract

ASJC Scopus subject areas

Physiology

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10.1016/0300-9629(74)90739-7

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title = "A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues",

abstract = "1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10-13 moles/ml of ATP can be repeatably and reliably detected.",

keywords = "ADP, ATP, chloride inhibition, firefly lantern extract",

author = "Silinsky, {Eugene M.}",

note = "Funding Information: Acknowledgements--My deepest thanks to Professor E. M. Margoliash and Dr. R. MacDonald for their useful criticism, and to Professor J. I. Hubbard and Peg Misk for their encouragement. I am also grateful to the BioMedical Engineering Department for allowing me the use of the machine shop to construct the reaction chamber. Part of this work was supported by an NSF grant to J. I. Hubbard.",

year = "1974",

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doi = "10.1016/0300-9629(74)90739-7",

language = "English (US)",

volume = "48",

pages = "561--571",

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TY - JOUR

T1 - A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues

AU - Silinsky, Eugene M.

N1 - Funding Information: Acknowledgements--My deepest thanks to Professor E. M. Margoliash and Dr. R. MacDonald for their useful criticism, and to Professor J. I. Hubbard and Peg Misk for their encouragement. I am also grateful to the BioMedical Engineering Department for allowing me the use of the machine shop to construct the reaction chamber. Part of this work was supported by an NSF grant to J. I. Hubbard.

PY - 1974/7/1

Y1 - 1974/7/1

N2 - 1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10-13 moles/ml of ATP can be repeatably and reliably detected.

AB - 1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10-13 moles/ml of ATP can be repeatably and reliably detected.

KW - ADP

KW - ATP

KW - chloride inhibition

KW - firefly lantern extract

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JO - Comparative Biochemistry and Physiology -- Part A: Physiology

JF - Comparative Biochemistry and Physiology -- Part A: Physiology

IS - 3

ER -

A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues

Abstract

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