A simple, rapid method for detecting the efflux of small quantities of adenosine triphosphate from biological tissues

Eugene M. Silinsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

1. 1. The entire light emission reaction that occurs upon injection of physiological saline solutions of nucleoside phosphates into commercial firefly lantern extract (FLE) was monitored, using a specially constructed reaction chamber fitted with a sensitive photomultiplier tube. 2. 2. Nucleoside phosphates were readily distinguished by the characteristic time course of their emission patterns, adenosine triphosphate (ATP) being the only nucleotide to give a sharply defined peak. ATP-adenosine diphosphate (ADP) mixtures were also distinguishable from pure solutions of either nucleotide. 3. 3. The first event that occurred after injection of ATP-saline was an immediate inhibition of the resting luminescence of the FLE, due predominantly to the chloride content of the saline. It is demonstrated that for accurate results this post-injection inhibition must be taken as the baseline for measurements of luminescence. 4. 4. Equations for precisely quantitating pure ATP solutions, pure ADP solutions and ATP-ADP mixtures are presented. 5. 5. Approximately 2 × 10-13 moles/ml of ATP can be repeatably and reliably detected.

Original languageEnglish (US)
Pages (from-to)561-571
Number of pages11
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume48
Issue number3
DOIs
StatePublished - Jul 1 1974

Keywords

  • ADP
  • ATP
  • chloride inhibition
  • firefly lantern extract

ASJC Scopus subject areas

  • Physiology

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