TY - JOUR
T1 - A simple, sensitive and widely applicable ELISA for S100B
T2 - Methodological features of the measurement of this glial protein
AU - Leite, Marina Concli
AU - Galland, Fabiana
AU - Brolese, Giovana
AU - Guerra, Maria Cristina
AU - Bortolotto, Josiane Woutheres
AU - Freitas, Rodrigo
AU - Almeida, Lucia Maria Vieira de
AU - Gottfried, Carmem
AU - Gonçalves, Carlos Alberto
N1 - Funding Information:
This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and FINEP/Rede IBN 01.06.0842-00. We would like to thank Ms. Alessandra Heizelmann for technical support with cell culture.
PY - 2008/3/30
Y1 - 2008/3/30
N2 - S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9 pg and 10 ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions.
AB - S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9 pg and 10 ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions.
KW - Adipocyte
KW - Astrocyte
KW - Brain injury
KW - ELISA
KW - Oxidative stress
KW - S100B protein
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U2 - 10.1016/j.jneumeth.2007.11.021
DO - 10.1016/j.jneumeth.2007.11.021
M3 - Article
C2 - 18178255
AN - SCOPUS:39749090021
SN - 0165-0270
VL - 169
SP - 93
EP - 99
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -