A small metalloribozyme with a two-step mechanism

Tao Pan*, Olke C. Uhlenbeck

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

231 Scopus citations


An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5′ cleavage product terminates with a 3′ phosphomonoester generated from a 2′,3′-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.

Original languageEnglish (US)
Pages (from-to)560-563
Number of pages4
Issue number6387
StatePublished - 1992

ASJC Scopus subject areas

  • General


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