Abstract
Multiparametric investigation of cardiac physiology is crucial for the diagnosis and therapy of heart disease. However, no method exists to simultaneously map multiple parameters that govern cardiac (patho)physiology from beating hearts in vivo. Here, we present a cardiac sensing platform that addresses this challenge, functioning with a wireless interface. Advanced fabrication and assembling strategies enable the heterogeneous integration of transparent microelectrodes, light-emitting diodes, photodiodes, and optical filters into a multilayer array structure on soft substrates. The microelectrodes exhibit superior electrochemical performance for measuring electrical potentials and excellent transparency for co-localized fluorescence measurement. The device shows excellent biocompatibility and records the fluorescence of calcium reporter with performance comparable to imaging cameras. Multiparametric in vivo mapping of electrical excitation, calcium dynamics, and their combined effects on cardiac excitation-contraction coupling is demonstrated during normal rhythm, arrhythmia, and treatment. This technology offers potential widespread use in cardiac research to support scientific discoveries and advance clinical life-saving diagnostics and therapies.
| Original language | English (US) |
|---|---|
| Article number | eads8608 |
| Journal | Science Advances |
| Volume | 11 |
| Issue number | 6 |
| DOIs | |
| State | Published - Feb 7 2025 |
Funding
this work was carried out in part at the George Washington University nanofabrication and imaging center. Metal analysis was performed at the northwestern University Quantitative Bio-element imaging center generously supported by nASA Ames Research center nnA06cB93G. histology services were provided by the northwestern University Mouse histology and Phenotyping laboratory, which is supported by nci P30-cA060553 awarded to the Robert h. lurie comprehensive cancer center. this work was supported by the national Science Foundation (grant nos. 2011093 to l.l. and i.R.e., 2131682 to l.l. and i.R.e., and 2339030 to l.l.) and by the national institutes of health (grant no. R01hl166746 to l.l.), grant no. R01hl141470 (to i.R.e.), and leducq Foundation Bioelectronics for neurocardiology (to i.R.e.).
ASJC Scopus subject areas
- General
