A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer; Martha A. Shaughnessy; Jacob J. Pruzansky

doi:10.1016/0022-1759(83)90262-4

A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer^*, Martha A. Shaughnessy, Jacob J. Pruzansky

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

Original language	English (US)
Pages (from-to)	49-57
Number of pages	9
Journal	Journal of Immunological Methods
Volume	58
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(83)90262-4
State	Published - Mar 11 1983

Keywords

antigen E
radioimmunoassay
ragweed
specific IgE

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(83)90262-4

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title = "A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E",

abstract = "A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.",

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T1 - A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

AU - Grammer, Leslie C.

AU - Shaughnessy, Martha A.

AU - Pruzansky, Jacob J.

PY - 1983/3/11

Y1 - 1983/3/11

N2 - A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

AB - A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

KW - antigen E

KW - radioimmunoassay

KW - ragweed

KW - specific IgE

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