A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer*, Martha A. Shaughnessy, Jacob J. Pruzansky

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalJournal of Immunological Methods
Volume58
Issue number1-2
DOIs
Publication statusPublished - Mar 11 1983

    Fingerprint

Keywords

  • antigen E
  • radioimmunoassay
  • ragweed
  • specific IgE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this