A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer; Martha A. Shaughnessy; Jacob J. Pruzansky

doi:10.1016/0022-1759(83)90262-4

A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer^*, Martha A. Shaughnessy, Jacob J. Pruzansky

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

Original language	English (US)
Pages (from-to)	49-57
Number of pages	9
Journal	Journal of Immunological Methods
Volume	58
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(83)90262-4
Publication status	Published - Mar 11 1983

Fingerprint

Keywords

antigen E
radioimmunoassay
ragweed
specific IgE

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Cite this

Grammer, L. C., Shaughnessy, M. A., & Pruzansky, J. J. (1983). A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E. Journal of Immunological Methods, 58(1-2), 49-57. https://doi.org/10.1016/0022-1759(83)90262-4

@article{a7b30e670ff3416fa982c64a65911e1c,

title = "A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E",

abstract = "A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.",

keywords = "antigen E, radioimmunoassay, ragweed, specific IgE",

author = "Grammer, {Leslie C.} and Shaughnessy, {Martha A.} and Pruzansky, {Jacob J.}",

year = "1983",

month = "3",

day = "11",

doi = "10.1016/0022-1759(83)90262-4",

language = "English (US)",

volume = "58",

pages = "49--57",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - A solid-phase bead radioimmunoassay for specific IgE to ragweed antigen E

AU - Grammer, Leslie C.

AU - Shaughnessy, Martha A.

AU - Pruzansky, Jacob J.

PY - 1983/3/11

Y1 - 1983/3/11

N2 - A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

AB - A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.

KW - antigen E

KW - radioimmunoassay

KW - ragweed

KW - specific IgE

UR - http://www.scopus.com/inward/record.url?scp=0020663785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020663785&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(83)90262-4

DO - 10.1016/0022-1759(83)90262-4

M3 - Article

C2 - 6339635

AN - SCOPUS:0020663785

VL - 58

SP - 49

EP - 57

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -

Access to Document

10.1016/0022-1759(83)90262-4