Abstract
A solid-phase bead radioimmunoassay has been developed for measuring IgE against antigen E (IgE-a-AgE) in serum. The patient's IgE is bound by monoclonal mouse anti-human IgE which is on the surface of a solid bead. Other immunoglobulin classes are not bound and can be washed away. Radiolabeled AgE is then added and is specifically bound by IgE-a-AgE. This assay is reproducible, and in contrast to specific IgE assays utilizing RAST methodology, it cannot be inhibited by specific antibody of other classes. Moreover, the solid-phase bead method does not utilize myeloma IgE. Finally, its results correlate well with those of a previously developed polystyrene tube method.
Original language | English (US) |
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Pages (from-to) | 49-57 |
Number of pages | 9 |
Journal | Journal of Immunological Methods |
Volume | 58 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 11 1983 |
Keywords
- antigen E
- radioimmunoassay
- ragweed
- specific IgE
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology