A specific sub-set of host-cell mRNAs prime influenza virus mRNA synthesis

Michael W. Shaw*, Robert A. Lamb

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The host-cell derived RNA primer sequences at the 5′ termini of mRNAs of influenza A and B viruses, obtained from sequences of 29 cDNA clones, have been compared. This has been done for clones of five different genome segments from four strains of influenza A and B virus. The results indicate that host RNA primers containing a 3′-terminal Py-G-C-A sequence before the presumed endonuclease cleavage site are preferred for use as primers in influenza virus mRNA synthesis. Primer-extension analyses of the 5′-terminal heterogeneous sequences of in vivo synthesized mRNAs confirm the preference for G-C-A-terminated primer fragments, with some differences noted between the transcripts of types A and B influenza virus genome segments.

Original languageEnglish (US)
Pages (from-to)455-467
Number of pages13
JournalVirus Research
Volume1
Issue number6
DOIs
StatePublished - Sep 1984

Keywords

  • host cell sequence
  • influenza virus transcription
  • mRNA analysis

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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