## Abstract

We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.

Original language | English (US) |
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Title of host publication | Computational Fluid and Solid Mechanics 2003 |

Publisher | Elsevier Inc |

Pages | 1790-1793 |

Number of pages | 4 |

ISBN (Electronic) | 9780080529479 |

ISBN (Print) | 9780080440460 |

DOIs | |

State | Published - Jun 2 2003 |

## Keywords

- Biological fluid dynamics
- Electron microscopy
- Extracellular matrix
- Quick-freeze/deep-etch
- Specific hydraulic conductivity
- Stereology

## ASJC Scopus subject areas

- Engineering(all)