Abstract
We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.
| Original language | English (US) |
|---|---|
| Title of host publication | Computational Fluid and Solid Mechanics 2003 |
| Publisher | Elsevier Inc |
| Pages | 1790-1793 |
| Number of pages | 4 |
| ISBN (Electronic) | 9780080529479 |
| ISBN (Print) | 9780080440460 |
| DOIs | |
| State | Published - Jun 2 2003 |
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Keywords
- Biological fluid dynamics
- Electron microscopy
- Extracellular matrix
- Quick-freeze/deep-etch
- Specific hydraulic conductivity
- Stereology
ASJC Scopus subject areas
- Engineering(all)
Cite this
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A stereologic technique to quantify the specific hydraulic conductivity of extracellular matrix using electron microscopy. / Overby, Darryl; Johnson, Mark.
Computational Fluid and Solid Mechanics 2003. Elsevier Inc, 2003. p. 1790-1793.Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - A stereologic technique to quantify the specific hydraulic conductivity of extracellular matrix using electron microscopy
AU - Overby, Darryl
AU - Johnson, Mark
PY - 2003/6/2
Y1 - 2003/6/2
N2 - We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.
AB - We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.
KW - Biological fluid dynamics
KW - Electron microscopy
KW - Extracellular matrix
KW - Quick-freeze/deep-etch
KW - Specific hydraulic conductivity
KW - Stereology
UR - http://www.scopus.com/inward/record.url?scp=84941661741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941661741&partnerID=8YFLogxK
U2 - 10.1016/B978-008044046-0.50437-1
DO - 10.1016/B978-008044046-0.50437-1
M3 - Chapter
SN - 9780080440460
SP - 1790
EP - 1793
BT - Computational Fluid and Solid Mechanics 2003
PB - Elsevier Inc
ER -