A stereologic technique to quantify the specific hydraulic conductivity of extracellular matrix using electron microscopy

Darryl Overby, Mark Johnson*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.

Original languageEnglish (US)
Title of host publicationComputational Fluid and Solid Mechanics 2003
PublisherElsevier Inc
Number of pages4
ISBN (Electronic)9780080529479
ISBN (Print)9780080440460
StatePublished - Jun 2 2003



  • Biological fluid dynamics
  • Electron microscopy
  • Extracellular matrix
  • Quick-freeze/deep-etch
  • Specific hydraulic conductivity
  • Stereology

ASJC Scopus subject areas

  • Engineering(all)

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