Abstract
We describe a novel stereologic technique to quantify the specific hydraulic conductivity, K, of extracellular matrix (ECM) directly from electron micrographs. This approach utilizes Darcy's law and Carmen-Kozeny theory to express K in terms of ECM porosity (s: ratio of void volume to total volume) and specific surface area (a: ratio of wetted surface area to total volume). A stereologic technique is developed to define s and of, and hence K, in terms of the measurable pore area and perimeter on a micrograph of known depth-of-field. Monte Carlo simulations of random polymer arrangements are performed to demonstrate the accuracy of the stereologic theory.
Original language | English (US) |
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Title of host publication | Computational Fluid and Solid Mechanics 2003 |
Publisher | Elsevier Inc |
Pages | 1790-1793 |
Number of pages | 4 |
ISBN (Electronic) | 9780080529479 |
ISBN (Print) | 9780080440460 |
DOIs | |
State | Published - Jun 2 2003 |
Keywords
- Biological fluid dynamics
- Electron microscopy
- Extracellular matrix
- Quick-freeze/deep-etch
- Specific hydraulic conductivity
- Stereology
ASJC Scopus subject areas
- Engineering(all)