@article{afb9a5a624314e0e88e9448e63e0e449,
title = "A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms",
abstract = "Structural analysis of an endogenous human SWI/SNF complex shows how it engages with nucleosomes and provides insight into how cancer-associated mutations affect the remodeler's function.",
keywords = "ATP-dependent chromatin remodeling, BAF complex, cancer, cross-linking mass spectrometry, cryoelectron microscopy, homology modeling, mammalian SWI/SNF complexes, mutations",
author = "Nazar Mashtalir and Hiroshi Suzuki and Farrell, {Daniel P.} and Akshay Sankar and Jie Luo and Martin Filipovski and D'Avino, {Andrew R.} and {St. Pierre}, Roodolph and Valencia, {Alfredo M.} and Takashi Onikubo and Roeder, {Robert G.} and Yan Han and Yuan He and Ranish, {Jeffrey A.} and Frank DiMaio and Thomas Walz and Cigall Kadoch",
note = "Funding Information: We thank members of the Kadoch, Walz, and DiMaio laboratories for their input and advice throughout this project, especially M. Bush (Walz Lab), as well as M. Ebrahim and J. Sotiris for help with grid screening and data collection at the Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center at The Rockefeller University. We also thank Z. Liu and D. Stokes at the Cryo-Electron Microscopy Laboratory at New York University Langone Health for support in data collection. This study was supported by the Pew-Stewart Scholars in Cancer Research Award (to C.K.), the American Cancer Society Research Scholar Award ( RSG-14-051-01-DMC to C.K.), and the NIH 1DP2CA195762-01 (to C.K.), K99CA237855 to N.M., R01CA234575 to R.G.R, the Simons Foundation Junior Fellowship (to T.O.) and R01GM110064 to J.A.R). Funding Information: We thank members of the Kadoch, Walz, and DiMaio laboratories for their input and advice throughout this project, especially M. Bush (Walz Lab), as well as M. Ebrahim and J. Sotiris for help with grid screening and data collection at the Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center at The Rockefeller University. We also thank Z. Liu and D. Stokes at the Cryo-Electron Microscopy Laboratory at New York University Langone Health for support in data collection. This study was supported by the Pew-Stewart Scholars in Cancer Research Award (to C.K.), the American Cancer Society Research Scholar Award (RSG-14-051-01-DMC to C.K.), and the NIH 1DP2CA195762-01 (to C.K.), K99CA237855 to N.M. R01CA234575 to R.G.R, the Simons Foundation Junior Fellowship (to T.O.) and R01GM110064 to J.A.R). N.M. H.S. T.W. and C.K. conceived of the study. N.M. H.S. J.L. and M.F. performed experiments. D.P.F. and F.D. performed all homology modeling. A.S. performed all analyses and mutational mapping. A.R.D. established computational methods and assisted in analyses. J.L. performed and analyzed all cross-linking mass spectrometry experiments, under supervision by J.A.R. R.S. and A.M.V. provided critical reagents and technical advice. T.O. generated and purified all nucleosomes used in the study, under supervision by R.G.R. All yeast SWI/SNF cryo-EM studies were performed by Y.H. and Y.H. C.K. T.W. and F.D. supervised the study. N.M. H.S. D.P.F. A.S. F.D. T.W. and C.K. wrote and edited the paper. C.K. is the scientific founder, fiduciary Board of Directors member, Scientific Advisory Board Member, shareholder, and consultant for Foghorn Therapeutics, Inc. (Cambridge, MA). The other authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = oct,
day = "29",
doi = "10.1016/j.cell.2020.09.051",
language = "English (US)",
volume = "183",
pages = "802--817.e24",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}
