A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae

Nicole L. Inniss; Travis J. Kochan; George Minasov; Zdzislaw Wawrzak; Changsoo Chang; Kemin Tan; Ludmilla Shuvalova; Olga Kiryukhina; Sergii Pshenychnyi; Ruiying Wu; Ivegeniia Dubrovska; Gyorgy Babnigg; Michael Endres; Wayne F. Anderson; Alan R. Hauser; Andrzej Joachimiak; Karla J.F. Satchell

doi:10.1128/mra.01013-22

A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae

Nicole L. Inniss, Travis J. Kochan, George Minasov, Zdzislaw Wawrzak, Changsoo Chang, Kemin Tan, Ludmilla Shuvalova, Olga Kiryukhina, Sergii Pshenychnyi, Ruiying Wu, Ivegeniia Dubrovska, Gyorgy Babnigg, Michael Endres, Wayne F. Anderson, Alan R. Hauser, Andrzej Joachimiak, Karla J.F. Satchell^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Klebsiella pneumoniae is a leading cause of antibiotic-resistant-associated deaths in the world. Here, we report the deposition of 14 structures of enzymes from both the core and accessory genomes of sequence type 23 (ST23) K1 hypervirulent K. pneumoniae.

Original language	English (US)
Journal	Microbiology Resource Announcements
Volume	12
Issue number	2
DOIs	https://doi.org/10.1128/mra.01013-22
State	Published - Feb 2023

Funding

We thank Lukasz Jaroszewski and Adam Godzik for bioinformatic assessment of all targets. This project has been funded in whole or in part with federal funds from the Department of Health and Human Services, NIH, and National Institute of Allergy and Infectious Diseases under contract no. HHSN272201700060C and 75N93022C00035 (to K.J.F.S.). This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The use of Structural Biology Center beamlines is supported by U.S. Department of Energy, Office of Biological and Environmental Research under contract DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (grant 085P1000817). T.J.K. was supported by the American Heart Association (grant 837089). Additional support was from the Chicago Biomedical Consortium Catalyst Award and NIH award K24 AI04831 (to A.R.H.).

ASJC Scopus subject areas

Immunology and Microbiology (miscellaneous)
Genetics
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/mra.01013-22

Cite this

Inniss, N. L., Kochan, T. J., Minasov, G., Wawrzak, Z., Chang, C., Tan, K., Shuvalova, L., Kiryukhina, O., Pshenychnyi, S., Wu, R., Dubrovska, I., Babnigg, G., Endres, M., Anderson, W. F., Hauser, A. R., Joachimiak, A., & Satchell, K. J. F. (2023). A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae. Microbiology Resource Announcements, 12(2). https://doi.org/10.1128/mra.01013-22

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title = "A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae",

abstract = "Klebsiella pneumoniae is a leading cause of antibiotic-resistant-associated deaths in the world. Here, we report the deposition of 14 structures of enzymes from both the core and accessory genomes of sequence type 23 (ST23) K1 hypervirulent K. pneumoniae.",

author = "Inniss, {Nicole L.} and Kochan, {Travis J.} and George Minasov and Zdzislaw Wawrzak and Changsoo Chang and Kemin Tan and Ludmilla Shuvalova and Olga Kiryukhina and Sergii Pshenychnyi and Ruiying Wu and Ivegeniia Dubrovska and Gyorgy Babnigg and Michael Endres and Anderson, {Wayne F.} and Hauser, {Alan R.} and Andrzej Joachimiak and Satchell, {Karla J.F.}",

note = "Funding Information: We thank Lukasz Jaroszewski and Adam Godzik for bioinformatic assessment of all targets. This project has been funded in whole or in part with federal funds from the Department of Health and Human Services, NIH, and National Institute of Allergy and Infectious Diseases under contract no. HHSN272201700060C and 75N93022C00035 (to K.J.F.S.). This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The use of Structural Biology Center beamlines is supported by U.S. Department of Energy, Office of Biological and Environmental Research under contract DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (grant 085P1000817). T.J.K. was supported by the American Heart Association (grant 837089). Additional support was from the Chicago Biomedical Consortium Catalyst Award and NIH award K24 AI04831 (to A.R.H.). Publisher Copyright: {\textcopyright} 2023 American Society for Microbiology. All rights reserved.",

year = "2023",

month = feb,

doi = "10.1128/mra.01013-22",

language = "English (US)",

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Inniss, NL, Kochan, TJ, Minasov, G, Wawrzak, Z, Chang, C, Tan, K, Shuvalova, L, Kiryukhina, O, Pshenychnyi, S, Wu, R, Dubrovska, I, Babnigg, G, Endres, M, Anderson, WF, Hauser, AR, Joachimiak, A & Satchell, KJF 2023, 'A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae', Microbiology Resource Announcements, vol. 12, no. 2. https://doi.org/10.1128/mra.01013-22

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AU - Kochan, Travis J.

AU - Minasov, George

AU - Wawrzak, Zdzislaw

AU - Chang, Changsoo

AU - Tan, Kemin

AU - Shuvalova, Ludmilla

AU - Kiryukhina, Olga

AU - Pshenychnyi, Sergii

AU - Wu, Ruiying

AU - Dubrovska, Ivegeniia

AU - Babnigg, Gyorgy

AU - Endres, Michael

AU - Anderson, Wayne F.

AU - Hauser, Alan R.

AU - Joachimiak, Andrzej

AU - Satchell, Karla J.F.

N1 - Funding Information: We thank Lukasz Jaroszewski and Adam Godzik for bioinformatic assessment of all targets. This project has been funded in whole or in part with federal funds from the Department of Health and Human Services, NIH, and National Institute of Allergy and Infectious Diseases under contract no. HHSN272201700060C and 75N93022C00035 (to K.J.F.S.). This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The use of Structural Biology Center beamlines is supported by U.S. Department of Energy, Office of Biological and Environmental Research under contract DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (grant 085P1000817). T.J.K. was supported by the American Heart Association (grant 837089). Additional support was from the Chicago Biomedical Consortium Catalyst Award and NIH award K24 AI04831 (to A.R.H.). Publisher Copyright: © 2023 American Society for Microbiology. All rights reserved.

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N2 - Klebsiella pneumoniae is a leading cause of antibiotic-resistant-associated deaths in the world. Here, we report the deposition of 14 structures of enzymes from both the core and accessory genomes of sequence type 23 (ST23) K1 hypervirulent K. pneumoniae.

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A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

1.52 Angstrom Resolution Crystal Structure of Transcriptional Regulator HdfR from Klebsiella pneumoniae

2.70 Angstrom Resolution Crystal Structure of Uracil Phosphoribosyl Transferase from Klebsiella pneumoniae

Crystal Structure of the Peptidoglycan Binding Domain of the Outer Membrane Protein (OmpA) from Klebsiella pneumoniae with bound D-alanine

Cite this

A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Datasets

1.52 Angstrom Resolution Crystal Structure of Transcriptional Regulator HdfR from Klebsiella pneumoniae

2.70 Angstrom Resolution Crystal Structure of Uracil Phosphoribosyl Transferase from Klebsiella pneumoniae

Crystal Structure of the Peptidoglycan Binding Domain of the Outer Membrane Protein (OmpA) from Klebsiella pneumoniae with bound D-alanine

Cite this