A synthetic TLR3 ligand mitigates profibrotic fibroblast responses by inducing autocrine IFN signaling

Feng Fang, Kohtaro Ooka, Xiaoyong Sun, Ruchi Shah, Swati Bhattacharyya, Jun Wei, John Varga*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Activation of TLR3 by exogenous microbial ligands or endogenous injury-associated ligands leads to production of type I IFN. Scleroderma patients with progressive skin fibrosis display an IFN-regulated gene signature, implicating TLR3 signaling in the disease. In this study, we show that TLR3 expression was detected on foreskin, adult skin, and lung fibroblasts, and TLR3 levels were significantly elevated in a subset of scleroderma skin biopsies. In explanted skin and lung fibroblasts, the synthetic TLR3 ligand polyinosinic-polycytidylic acid (poly(I:C)), a dsRNA analog, caused dose- and time-dependent stimulation of IFN-b production and generation of an IFN-response gene signature that was accompanied by substantial downregulation of collagen and a-smooth muscle actin gene expression. Furthermore, poly(I:C) abrogated TGF-b-induced fibrotic responses and blocked canonical Smad signaling via upregulation of inhibitory Smad7. Surprisingly, the inhibitory effects of poly(I:C) in fibroblasts were independent of TLR3 and were mediated by the cytosolic receptors retinoic acid-inducible gene 1 and melanoma differentiation-associated gene 5, and involved signaling via the IFN receptor. Taken together, these results demonstrate that induction of a fibroblast IFN response gene signature triggered by dsRNA is associated with potent TLR3-independent anti-fibrotic effects. The characteristic IFN response gene signature seen in scleroderma lesions might therefore signify a tissue-autonomous protective attempt to restrict fibroblast activation during injury.

Original languageEnglish (US)
Pages (from-to)2956-2966
Number of pages11
JournalJournal of Immunology
Volume191
Issue number6
DOIs
StatePublished - Sep 15 2013

Fingerprint

Autocrine Communication
Fibroblasts
Ligands
Genes
Poly C
Skin
Foreskin
Poly I-C
Lung
Retinoic Acid Receptors
Wounds and Injuries
Smooth Muscle
Actins
Melanoma
Fibrosis
Up-Regulation
Collagen
Down-Regulation
Biopsy
Gene Expression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fang, Feng ; Ooka, Kohtaro ; Sun, Xiaoyong ; Shah, Ruchi ; Bhattacharyya, Swati ; Wei, Jun ; Varga, John. / A synthetic TLR3 ligand mitigates profibrotic fibroblast responses by inducing autocrine IFN signaling. In: Journal of Immunology. 2013 ; Vol. 191, No. 6. pp. 2956-2966.
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abstract = "Activation of TLR3 by exogenous microbial ligands or endogenous injury-associated ligands leads to production of type I IFN. Scleroderma patients with progressive skin fibrosis display an IFN-regulated gene signature, implicating TLR3 signaling in the disease. In this study, we show that TLR3 expression was detected on foreskin, adult skin, and lung fibroblasts, and TLR3 levels were significantly elevated in a subset of scleroderma skin biopsies. In explanted skin and lung fibroblasts, the synthetic TLR3 ligand polyinosinic-polycytidylic acid (poly(I:C)), a dsRNA analog, caused dose- and time-dependent stimulation of IFN-b production and generation of an IFN-response gene signature that was accompanied by substantial downregulation of collagen and a-smooth muscle actin gene expression. Furthermore, poly(I:C) abrogated TGF-b-induced fibrotic responses and blocked canonical Smad signaling via upregulation of inhibitory Smad7. Surprisingly, the inhibitory effects of poly(I:C) in fibroblasts were independent of TLR3 and were mediated by the cytosolic receptors retinoic acid-inducible gene 1 and melanoma differentiation-associated gene 5, and involved signaling via the IFN receptor. Taken together, these results demonstrate that induction of a fibroblast IFN response gene signature triggered by dsRNA is associated with potent TLR3-independent anti-fibrotic effects. The characteristic IFN response gene signature seen in scleroderma lesions might therefore signify a tissue-autonomous protective attempt to restrict fibroblast activation during injury.",
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A synthetic TLR3 ligand mitigates profibrotic fibroblast responses by inducing autocrine IFN signaling. / Fang, Feng; Ooka, Kohtaro; Sun, Xiaoyong; Shah, Ruchi; Bhattacharyya, Swati; Wei, Jun; Varga, John.

In: Journal of Immunology, Vol. 191, No. 6, 15.09.2013, p. 2956-2966.

Research output: Contribution to journalArticle

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