TY - JOUR
T1 - A three-state mathematical model of hyperthermic cell death
AU - O'Neill, David P.
AU - Peng, Tingying
AU - Stiegler, Philipp
AU - Mayrhauser, Ursula
AU - Koestenbauer, Sonja
AU - Tscheliessnigg, Karlheinz
AU - Payne, Stephen J.
N1 - Funding Information:
Katja Schick for her contributions to work in the cell culture. The research leading to these results has received funding from the European Community’s Seventh framework Programme under grant agreement no. 223877, project IMPPACT.
PY - 2011/1
Y1 - 2011/1
N2 - Thermal treatments for tissue ablation rely upon the heating of cells past a threshold beyond which the cells are considered destroyed, denatured, or killed. In this article, a novel three-state model for cell death is proposed where there exists a vulnerable state positioned between the alive and dead states used in a number of existing cell death models. Proposed rate coefficients include temperature dependence and the model is fitted to experimental data of heated co-cultures of hepatocytes and lung fibroblasts with very small RMS error. The experimental data utilized include further reductions in cell viabilities over 24 and 48 h post-heating and these data are used to extend the three-state model to account for slow cell death. For the two cell lines employed in the experimental data, the three parameters for fast cell death appear to be linearly increasing with % content of lung fibroblast, while the sparse nature of the data did not indicate any co-culture make-up dependence for the parameters for slow cell death. A critical post-heating cell viability threshold is proposed beyond which cells progress to death; and these results are of practical importance with potential for more accurate prediction of cell death.
AB - Thermal treatments for tissue ablation rely upon the heating of cells past a threshold beyond which the cells are considered destroyed, denatured, or killed. In this article, a novel three-state model for cell death is proposed where there exists a vulnerable state positioned between the alive and dead states used in a number of existing cell death models. Proposed rate coefficients include temperature dependence and the model is fitted to experimental data of heated co-cultures of hepatocytes and lung fibroblasts with very small RMS error. The experimental data utilized include further reductions in cell viabilities over 24 and 48 h post-heating and these data are used to extend the three-state model to account for slow cell death. For the two cell lines employed in the experimental data, the three parameters for fast cell death appear to be linearly increasing with % content of lung fibroblast, while the sparse nature of the data did not indicate any co-culture make-up dependence for the parameters for slow cell death. A critical post-heating cell viability threshold is proposed beyond which cells progress to death; and these results are of practical importance with potential for more accurate prediction of cell death.
KW - Cell death
KW - Hyperthermia
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U2 - 10.1007/s10439-010-0177-1
DO - 10.1007/s10439-010-0177-1
M3 - Article
C2 - 20924678
AN - SCOPUS:78650872259
SN - 0090-6964
VL - 39
SP - 570
EP - 579
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 1
ER -