Abstract
Herein, we describe the detailed synthesis of Ag/Au core-shell nanoparticles, the surface-functionalization of these particles with thiolated oligonucleotides, and their subsequent use as probes for DNA detection. The Ag/Au core-shell nanoparticles retain the optical properties of the silver core and are easily functionalized with thiolated oligonucleotides due to the presence of the gold shell. As such, the Ag/Au core-shell nanoparticles have optical properties different from their pure gold counterparts and provide another "color" option for target DNA-directed colorimetric detection. Size-matched Ag/Au core-shell and pure gold nanoparticles perform nearly identically in DNA detection and melting experiments, but with distinct optical signatures. Based on this observation, we report the development of a two-color-change method for the detection and simultaneous validation of single-nucleotide polymorphisms in a DNA target using Ag/Au core-shell and pure gold nanoparticle probes.
Original language | English (US) |
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Pages (from-to) | 449-455 |
Number of pages | 7 |
Journal | Talanta |
Volume | 67 |
Issue number | 3 |
DOIs | |
State | Published - Sep 15 2005 |
Keywords
- Core-shell
- Detection
- Nanoparticles
- Oligonucleotides
- Two-color-change
ASJC Scopus subject areas
- Analytical Chemistry