A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears

Iram Shahzadi; Safia Janjua; Fakhar Abbas; Gary J. Galbreath

doi:10.2192/URSUS-D-13-00014.1

A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears

Iram Shahzadi^*, Safia Janjua, Fakhar Abbas, Gary J. Galbreath

^*Corresponding author for this work

Program in Biological Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n = 12) and Himalayan brown (U. arctos; n = 6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83-92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.

Original language	English (US)
Pages (from-to)	73-77
Number of pages	5
Journal	Ursus
Volume	25
Issue number	1
DOIs	https://doi.org/10.2192/URSUS-D-13-00014.1
State	Published - May 1 2014

Keywords

Asiatic black bear
DNA barcoding
Himalayan brown bear
Ursidae
Ursus arctos
Ursus thibetanus
cytochrome c oxidase subunit I (COI) gene

ASJC Scopus subject areas

Animal Science and Zoology
Nature and Landscape Conservation
Management, Monitoring, Policy and Law

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10.2192/URSUS-D-13-00014.1

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title = "A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears",

abstract = "Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n = 12) and Himalayan brown (U. arctos; n = 6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83-92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.",

keywords = "Asiatic black bear, DNA barcoding, Himalayan brown bear, Ursidae, Ursus arctos, Ursus thibetanus, cytochrome c oxidase subunit I (COI) gene",

author = "Iram Shahzadi and Safia Janjua and Fakhar Abbas and Galbreath, {Gary J.}",

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T1 - A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears

AU - Shahzadi, Iram

AU - Janjua, Safia

AU - Abbas, Fakhar

AU - Galbreath, Gary J.

N1 - Publisher Copyright: © International Association for Bear Research and Management.

PY - 2014/5/1

Y1 - 2014/5/1

N2 - Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n = 12) and Himalayan brown (U. arctos; n = 6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83-92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.

AB - Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n = 12) and Himalayan brown (U. arctos; n = 6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83-92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.

KW - Asiatic black bear

KW - DNA barcoding

KW - Himalayan brown bear

KW - Ursidae

KW - Ursus arctos

KW - Ursus thibetanus

KW - cytochrome c oxidase subunit I (COI) gene

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DO - 10.2192/URSUS-D-13-00014.1

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VL - 25

SP - 73

EP - 77

JO - Ursus

JF - Ursus

IS - 1

ER -

A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears

Abstract

Keywords

ASJC Scopus subject areas

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