Ability of implants of polymer-entrapped antiprogesterone antiserum to absorb and deplete progesterone from serum of the pregnant rat

K. L. Cheesman, R. T. Chatterton, R. R. Mehta, D. L. Venton

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A highly specific antiprogesterone antiserum (APA) produced by immunization of sheep with an 11α-hydroxyprogesterone hemisuccinate-thyroglobulin conjugate was purified, and the IgG fraction was entrapped within a polysiloxane matrix. The matrix immobilized APA but allowed penetration and binding of progesterone (P) to the APA. In this entrapped form APA implanted intraperitoneally in rats on the tenth day of pregnancy resulted in a decline in serum P from 50 to 12 ng/ml within 12 hours and to less than 2 ng/ml within 36 hours. Free serum P measured by equilibrium dialysis fell to less than 0.2 ng/ml at 36 hours. Concomitant with the decline in serum P was a rise in both serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and eventual fetal resorption.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalFertility and Sterility
Volume38
Issue number4
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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