Ability of implants of polymer-entrapped antiprogesterone antiserum to absorb and deplete progesterone from serum of the pregnant rat

K. L. Cheesman; R. T. Chatterton; R. R. Mehta; D. L. Venton

doi:10.1016/s0015-0282(16)46584-2

Ability of implants of polymer-entrapped antiprogesterone antiserum to absorb and deplete progesterone from serum of the pregnant rat

K. L. Cheesman, R. T. Chatterton, R. R. Mehta, D. L. Venton

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A highly specific antiprogesterone antiserum (APA) produced by immunization of sheep with an 11α-hydroxyprogesterone hemisuccinate-thyroglobulin conjugate was purified, and the IgG fraction was entrapped within a polysiloxane matrix. The matrix immobilized APA but allowed penetration and binding of progesterone (P) to the APA. In this entrapped form APA implanted intraperitoneally in rats on the tenth day of pregnancy resulted in a decline in serum P from 50 to 12 ng/ml within 12 hours and to less than 2 ng/ml within 36 hours. Free serum P measured by equilibrium dialysis fell to less than 0.2 ng/ml at 36 hours. Concomitant with the decline in serum P was a rise in both serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and eventual fetal resorption.

Original language	English (US)
Pages (from-to)	475-481
Number of pages	7
Journal	Fertility and Sterility
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/s0015-0282(16)46584-2
State	Published - 1982

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/s0015-0282(16)46584-2

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title = "Ability of implants of polymer-entrapped antiprogesterone antiserum to absorb and deplete progesterone from serum of the pregnant rat",

abstract = "A highly specific antiprogesterone antiserum (APA) produced by immunization of sheep with an 11α-hydroxyprogesterone hemisuccinate-thyroglobulin conjugate was purified, and the IgG fraction was entrapped within a polysiloxane matrix. The matrix immobilized APA but allowed penetration and binding of progesterone (P) to the APA. In this entrapped form APA implanted intraperitoneally in rats on the tenth day of pregnancy resulted in a decline in serum P from 50 to 12 ng/ml within 12 hours and to less than 2 ng/ml within 36 hours. Free serum P measured by equilibrium dialysis fell to less than 0.2 ng/ml at 36 hours. Concomitant with the decline in serum P was a rise in both serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and eventual fetal resorption.",

author = "Cheesman, {K. L.} and Chatterton, {R. T.} and Mehta, {R. R.} and Venton, {D. L.}",

note = "Funding Information: Received February 22, 1982; revised and accepted June 14, 1982. *Presented in part at the Thirty-Seventh Annual Meeting of The American Fertility Society, March 14 to 18, 1981, Atlanta, Georgia; and supported by subc.ontract 203 from the Program for Applied Research in Fertility Regulation. tReproductive Endocrinology Laboratory, Department of Obstetrics and Gynecology, Northwestern University Medical School. :j:Reprint requests: Kerry L. Cheesman, Ph.D., Reproductive Endocrinology Laboratory, Department of Obstetrics and Gynecology, Northwestern University Medical School, 333 East Superior Street, Chicago, Illinois 60611. §Department of Medicinal Chemistry, University of Illinois at the Medical Center.",

year = "1982",

doi = "10.1016/s0015-0282(16)46584-2",

language = "English (US)",

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AU - Mehta, R. R.

AU - Venton, D. L.

N1 - Funding Information: Received February 22, 1982; revised and accepted June 14, 1982. *Presented in part at the Thirty-Seventh Annual Meeting of The American Fertility Society, March 14 to 18, 1981, Atlanta, Georgia; and supported by subc.ontract 203 from the Program for Applied Research in Fertility Regulation. tReproductive Endocrinology Laboratory, Department of Obstetrics and Gynecology, Northwestern University Medical School. :j:Reprint requests: Kerry L. Cheesman, Ph.D., Reproductive Endocrinology Laboratory, Department of Obstetrics and Gynecology, Northwestern University Medical School, 333 East Superior Street, Chicago, Illinois 60611. §Department of Medicinal Chemistry, University of Illinois at the Medical Center.

PY - 1982

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N2 - A highly specific antiprogesterone antiserum (APA) produced by immunization of sheep with an 11α-hydroxyprogesterone hemisuccinate-thyroglobulin conjugate was purified, and the IgG fraction was entrapped within a polysiloxane matrix. The matrix immobilized APA but allowed penetration and binding of progesterone (P) to the APA. In this entrapped form APA implanted intraperitoneally in rats on the tenth day of pregnancy resulted in a decline in serum P from 50 to 12 ng/ml within 12 hours and to less than 2 ng/ml within 36 hours. Free serum P measured by equilibrium dialysis fell to less than 0.2 ng/ml at 36 hours. Concomitant with the decline in serum P was a rise in both serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and eventual fetal resorption.

AB - A highly specific antiprogesterone antiserum (APA) produced by immunization of sheep with an 11α-hydroxyprogesterone hemisuccinate-thyroglobulin conjugate was purified, and the IgG fraction was entrapped within a polysiloxane matrix. The matrix immobilized APA but allowed penetration and binding of progesterone (P) to the APA. In this entrapped form APA implanted intraperitoneally in rats on the tenth day of pregnancy resulted in a decline in serum P from 50 to 12 ng/ml within 12 hours and to less than 2 ng/ml within 36 hours. Free serum P measured by equilibrium dialysis fell to less than 0.2 ng/ml at 36 hours. Concomitant with the decline in serum P was a rise in both serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and eventual fetal resorption.

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Ability of implants of polymer-entrapped antiprogesterone antiserum to absorb and deplete progesterone from serum of the pregnant rat

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