Abnormal scar identification with sphericalnucleic-acid technology

David C. Yeo, Christian Wiraja, Amy S. Paller*, Chad A. Mirkin, Chenjie Xu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


The accurate diagnosis of scar type and severity relies on histopathology of biopsied tissue, which is invasive and time-consuming, causes discomfort and may exacerbate scarring. Here, we show that imaging nanoprobes for the live-cell detection of intracellular messenger RNA (mRNA) (also known as NanoFlares) enable measurements of the expression of connective tissue growth factor (CTGF) as a visual indicator of hypertrophic scars and keloids. During cell culture, NanoFlares enabled the distinction of hypertrophic and keloidal fibroblasts from normal fibroblasts, and the detection of changes in CTGF expression resulting from the regulatory effects of transforming growth factor-β (TGF-β) agonists and TGF-β antagonists. We also applied the NanoFlares topically to the skin of live mice and rabbits, and to ex vivo human skin models. Transepidermal penetration of the NanoFlares enabled the visual and spectroscopic quantification of underlying abnormal fibroblasts on the basis of CTGF mRNA expression. Our proof-of-concept studies of topically applied NanoFlare technology as a means of biopsy-free scar diagnosis may eventually inform therapeutic decisions on the basis of the mRNA-expression patterns of skin disorders.

Original languageEnglish (US)
Pages (from-to)227-238
Number of pages12
JournalNature Biomedical Engineering
Issue number4
StatePublished - Apr 1 2018

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Computer Science Applications


Dive into the research topics of 'Abnormal scar identification with sphericalnucleic-acid technology'. Together they form a unique fingerprint.

Cite this