Absence of a stratum corneum antigen in disorders of epidermal cell proliferation: Detection with an anti-ganglioside GM3 antibody

Amy S. Paller*, Joan N. Siegel, Douglas E. Spalding, Eric G. Bremer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We have investigated the distribution of ganglioside GM3 in normal skin with 8G9D8, a monoclonal antibody against GM3, and found binding to the stratum corneum. By electron microscopy, strong deposition of antibody was seen at the corneocyte envelope/"plasma membrane" region. Significantly decreased to absent binding to the stratum corneum was shown in a variety of disorders of excessive keratinocyte proliferation, including squamous cell carcinomas, psoriasis, and bullous and non-bullous forms of congenital ichthyosiform erythrodermas, as well as in the hyperplastic cornoid lamellae of porokeratosis. The 8G9D8 antibody recognizes the carbohydrate sequence N-acetylneuraminic acid α2 → 3 galactose βl → 4 glucose (or N-acetylglucosamine). Thus, in addition to ganglioside GM3, 8G9D8 may bind to glycoproteins or another glycolipid of the stratum corneum with a shared carbohydrate sequence. The carbohydrate sequence recognized by 8G9D8, whether attached to an epidermal glycoprotein or glycolipid, may prove to be important in keratinocyte proliferation and differentiation.

Original languageEnglish (US)
Pages (from-to)240-246
Number of pages7
JournalJournal of Investigative Dermatology
Volume92
Issue number2
DOIs
StatePublished - Feb 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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