Accelerometer-measured Physical Activity, Reproductive Hormones, and DNA Methylation

Y. U.E. Wu; Jaclyn M. Goodrich; Dana C. Dolinoy; Brisa N. Sánchez; Edward A. Ruiz-Narváez; Margaret Banker; Alejandra Cantoral; Adriana Mercado-Garcia; Martha M. Téllez-Rojo; Karen E. Peterson

doi:10.1249/MSS.0000000000002175

Accelerometer-measured Physical Activity, Reproductive Hormones, and DNA Methylation

Y. U.E. Wu, Jaclyn M. Goodrich, Dana C. Dolinoy, Brisa N. Sánchez, Edward A. Ruiz-Narváez, Margaret Banker, Alejandra Cantoral, Adriana Mercado-Garcia, Martha M. Téllez-Rojo, Karen E. Peterson^*

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Introduction/Purpose Limited studies have examined the association of physical activity with reproductive hormones, DNA methylation, and pubertal status among adolescents. Methods Among 248 boys and 271 girls, we estimated daily physical activity levels based on 7 d of wrist-worn accelerometer data. We used an isotemporal substitution paradigm and sex-stratified regression models to examine the association of physical activity levels with 1) testosterone, cortisol, progesterone, and androstenedione concentrations; 2) DNA methylation of long interspersed nucleotide (LINE-1) repeats and the genes H19, hydroxysteroid (11-Beta) dehydrogenase 2 (HSD11B2), and peroxisome proliferator-activated receptor alpha (PPARA) from blood leukocytes; and 3) Tanner stages, adjusted for age, BMI, and socioeconomic status. Results In boys, substituting 30 min of moderate physical activity for 30 min of sedentary behavior per day was associated with 29% (-49%, 0%) of lower testosterone and 29% (4%, 61%) of higher progesterone. Substituting 30 min of light physical activity for sedentary behavior was associated with 13% (-22%, -2%) of lower progesterone. Among girls, 30 min of additional sedentary behavior was associated with 8% (-15%, 0%) of lower testosterone and 24% (8%, 42%) of higher progesterone concentrations. Substituting 30 min of moderate physical activity for sedentary behavior was associated with 15% (0%, 31%) of higher cortisol, whereas substituting the same amount of light physical activity for sedentary behavior was associated with 22% (-39%, 0%) of lower progesterone. Substituting 30 min of vigorous physical activity for sedentary behavior per day was associated with almost six times higher levels (5.83, 95% confidence interval = 1.79-9.86) of HSD11B2 methylation in boys. Conclusions Accelerometer-measured daily physical activity was associated with reproductive hormones and HSD11B2 DNA methylation, differed by sex and activity intensity levels.

Original language	English (US)
Pages (from-to)	598-607
Number of pages	10
Journal	Medicine and science in sports and exercise
Volume	52
Issue number	3
DOIs	https://doi.org/10.1249/MSS.0000000000002175
State	Published - Mar 1 2020

Funding

The authors acknowledge the research staff at participating hospitals and the American British Cowdray Hospital in Mexico City for providing facilities used for this research. They thank the mothers and children for participating in the study. This work was supported by the U.S. Environmental Protection Agency (EPA) grant nos. RD834800 and RD83543601 and the National Institute for Environmental Health Sciences grant nos. P20 ES018171, P01 ES02284401, R01 ES007821, R01 ES014930, R01 ES013744, and P30 ES017885. This study was also supported and partially funded by the National Institute of Public Health/Ministry of Health of Mexico. The contents of this publication are solely the responsibility of the grantee and do not necessarily represent the official views of the U.S. EPA or the NIH. Further, the U.S. EPA does not endorse the purchase of any commercial products or services mentioned in the publication.

Keywords

Accelerometer
DNA methylation
Physical Activity
Reproductive Hormones
Sexual Maturation

ASJC Scopus subject areas

Orthopedics and Sports Medicine
Physical Therapy, Sports Therapy and Rehabilitation

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10.1249/MSS.0000000000002175

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Wu, Y. U. E., Goodrich, J. M., Dolinoy, D. C., Sánchez, B. N., Ruiz-Narváez, E. A., Banker, M., Cantoral, A., Mercado-Garcia, A., Téllez-Rojo, M. M., & Peterson, K. E. (2020). Accelerometer-measured Physical Activity, Reproductive Hormones, and DNA Methylation. Medicine and science in sports and exercise, 52(3), 598-607. https://doi.org/10.1249/MSS.0000000000002175

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abstract = "Introduction/Purpose Limited studies have examined the association of physical activity with reproductive hormones, DNA methylation, and pubertal status among adolescents. Methods Among 248 boys and 271 girls, we estimated daily physical activity levels based on 7 d of wrist-worn accelerometer data. We used an isotemporal substitution paradigm and sex-stratified regression models to examine the association of physical activity levels with 1) testosterone, cortisol, progesterone, and androstenedione concentrations; 2) DNA methylation of long interspersed nucleotide (LINE-1) repeats and the genes H19, hydroxysteroid (11-Beta) dehydrogenase 2 (HSD11B2), and peroxisome proliferator-activated receptor alpha (PPARA) from blood leukocytes; and 3) Tanner stages, adjusted for age, BMI, and socioeconomic status. Results In boys, substituting 30 min of moderate physical activity for 30 min of sedentary behavior per day was associated with 29\% (-49\%, 0\%) of lower testosterone and 29\% (4\%, 61\%) of higher progesterone. Substituting 30 min of light physical activity for sedentary behavior was associated with 13\% (-22\%, -2\%) of lower progesterone. Among girls, 30 min of additional sedentary behavior was associated with 8\% (-15\%, 0\%) of lower testosterone and 24\% (8\%, 42\%) of higher progesterone concentrations. Substituting 30 min of moderate physical activity for sedentary behavior was associated with 15\% (0\%, 31\%) of higher cortisol, whereas substituting the same amount of light physical activity for sedentary behavior was associated with 22\% (-39\%, 0\%) of lower progesterone. Substituting 30 min of vigorous physical activity for sedentary behavior per day was associated with almost six times higher levels (5.83, 95\% confidence interval = 1.79-9.86) of HSD11B2 methylation in boys. Conclusions Accelerometer-measured daily physical activity was associated with reproductive hormones and HSD11B2 DNA methylation, differed by sex and activity intensity levels.",

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AU - Wu, Y. U.E.

AU - Goodrich, Jaclyn M.

AU - Dolinoy, Dana C.

AU - Sánchez, Brisa N.

AU - Ruiz-Narváez, Edward A.

AU - Banker, Margaret

AU - Cantoral, Alejandra

AU - Mercado-Garcia, Adriana

AU - Téllez-Rojo, Martha M.

AU - Peterson, Karen E.

N1 - Publisher Copyright: © Lippincott Williams & Wilkins.

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Y1 - 2020/3/1

N2 - Introduction/Purpose Limited studies have examined the association of physical activity with reproductive hormones, DNA methylation, and pubertal status among adolescents. Methods Among 248 boys and 271 girls, we estimated daily physical activity levels based on 7 d of wrist-worn accelerometer data. We used an isotemporal substitution paradigm and sex-stratified regression models to examine the association of physical activity levels with 1) testosterone, cortisol, progesterone, and androstenedione concentrations; 2) DNA methylation of long interspersed nucleotide (LINE-1) repeats and the genes H19, hydroxysteroid (11-Beta) dehydrogenase 2 (HSD11B2), and peroxisome proliferator-activated receptor alpha (PPARA) from blood leukocytes; and 3) Tanner stages, adjusted for age, BMI, and socioeconomic status. Results In boys, substituting 30 min of moderate physical activity for 30 min of sedentary behavior per day was associated with 29% (-49%, 0%) of lower testosterone and 29% (4%, 61%) of higher progesterone. Substituting 30 min of light physical activity for sedentary behavior was associated with 13% (-22%, -2%) of lower progesterone. Among girls, 30 min of additional sedentary behavior was associated with 8% (-15%, 0%) of lower testosterone and 24% (8%, 42%) of higher progesterone concentrations. Substituting 30 min of moderate physical activity for sedentary behavior was associated with 15% (0%, 31%) of higher cortisol, whereas substituting the same amount of light physical activity for sedentary behavior was associated with 22% (-39%, 0%) of lower progesterone. Substituting 30 min of vigorous physical activity for sedentary behavior per day was associated with almost six times higher levels (5.83, 95% confidence interval = 1.79-9.86) of HSD11B2 methylation in boys. Conclusions Accelerometer-measured daily physical activity was associated with reproductive hormones and HSD11B2 DNA methylation, differed by sex and activity intensity levels.

AB - Introduction/Purpose Limited studies have examined the association of physical activity with reproductive hormones, DNA methylation, and pubertal status among adolescents. Methods Among 248 boys and 271 girls, we estimated daily physical activity levels based on 7 d of wrist-worn accelerometer data. We used an isotemporal substitution paradigm and sex-stratified regression models to examine the association of physical activity levels with 1) testosterone, cortisol, progesterone, and androstenedione concentrations; 2) DNA methylation of long interspersed nucleotide (LINE-1) repeats and the genes H19, hydroxysteroid (11-Beta) dehydrogenase 2 (HSD11B2), and peroxisome proliferator-activated receptor alpha (PPARA) from blood leukocytes; and 3) Tanner stages, adjusted for age, BMI, and socioeconomic status. Results In boys, substituting 30 min of moderate physical activity for 30 min of sedentary behavior per day was associated with 29% (-49%, 0%) of lower testosterone and 29% (4%, 61%) of higher progesterone. Substituting 30 min of light physical activity for sedentary behavior was associated with 13% (-22%, -2%) of lower progesterone. Among girls, 30 min of additional sedentary behavior was associated with 8% (-15%, 0%) of lower testosterone and 24% (8%, 42%) of higher progesterone concentrations. Substituting 30 min of moderate physical activity for sedentary behavior was associated with 15% (0%, 31%) of higher cortisol, whereas substituting the same amount of light physical activity for sedentary behavior was associated with 22% (-39%, 0%) of lower progesterone. Substituting 30 min of vigorous physical activity for sedentary behavior per day was associated with almost six times higher levels (5.83, 95% confidence interval = 1.79-9.86) of HSD11B2 methylation in boys. Conclusions Accelerometer-measured daily physical activity was associated with reproductive hormones and HSD11B2 DNA methylation, differed by sex and activity intensity levels.

KW - Accelerometer

KW - DNA methylation

KW - Physical Activity

KW - Reproductive Hormones

KW - Sexual Maturation

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Accelerometer-measured Physical Activity, Reproductive Hormones, and DNA Methylation

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