TY - JOUR
T1 - Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae
AU - Chen, Chuyi
AU - Gu, Yuyang
AU - Philippe, Julien
AU - Zhang, Peiran
AU - Bachman, Hunter
AU - Zhang, Jinxin
AU - Mai, John
AU - Rufo, Joseph
AU - Rawls, John F.
AU - Davis, Erica E.
AU - Katsanis, Elias Nicholas
AU - Huang, Tony Jun
N1 - Funding Information:
We acknowledge support from the National Institutes of Health (UG3TR002978, R01GM132603, R01GM135486, R33CA223908, R01GM127714, and R43OD024963), the United States Army Medical Research Acquisition Activity (W81XWH-18-1-0242), and the National Science Foundation (ECCS-1807601). We also acknowledge support from the Zebrafish Core Facility (Z-Core) and the Shared Materials Instrumentation Facility (SMIF) at Duke University.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (~1 s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.
AB - Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (~1 s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.
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U2 - 10.1038/s41467-021-21373-3
DO - 10.1038/s41467-021-21373-3
M3 - Article
C2 - 33602914
AN - SCOPUS:85101193437
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1118
ER -
