Actin dependent CD95 internalization is specific for Type I cells

Alicia Algeciras-Schimnich; Marcus E. Peter

doi:10.1016/S0014-5793(03)00558-1

Actin dependent CD95 internalization is specific for Type I cells

Alicia Algeciras-Schimnich, Marcus E. Peter^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

CD95 Type I and Type II cells differ in the requirement for mitochondrial contribution to the execution of apoptosis. Mitochondria are required to amplify the apoptosis signal only in Type II cells. Here we show that CD95 internalizes in an actin dependent manner only in Type I cells and that the two CD95 apoptosis cell types differ in their threshold of active caspase-8 required to execute apoptosis. The data suggest that CD95 is linked to the cytoskeleton in different ways in the two cell types and that they have adapted to different levels of active caspase-8 generated at the activated receptor.

Original language	English (US)
Pages (from-to)	185-188
Number of pages	4
Journal	FEBS Letters
Volume	546
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00558-1
State	Published - Jul 10 2003

Keywords

Actin
Apoptosis
Caspase-8
Endocytosis
Fas

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)00558-1

Cite this

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title = "Actin dependent CD95 internalization is specific for Type I cells",

abstract = "CD95 Type I and Type II cells differ in the requirement for mitochondrial contribution to the execution of apoptosis. Mitochondria are required to amplify the apoptosis signal only in Type II cells. Here we show that CD95 internalizes in an actin dependent manner only in Type I cells and that the two CD95 apoptosis cell types differ in their threshold of active caspase-8 required to execute apoptosis. The data suggest that CD95 is linked to the cytoskeleton in different ways in the two cell types and that they have adapted to different levels of active caspase-8 generated at the activated receptor.",

keywords = "Actin, Apoptosis, Caspase-8, Endocytosis, Fas",

author = "Alicia Algeciras-Schimnich and Peter, {Marcus E.}",

note = "Funding Information: We like to thank Dr. P. Krammer for providing us with anti-APO-1 and Dr. H. Walczak for his generous gift of LzCD95L. We would also like to thank E. Pietras for excellent technical assistance, S. Vijayan for performing the HS1 Western blot, and B. Barnhart for critically reading the manuscript. This work has been funded by the NIH Grant GM61712. A.A.-S. was supported by the Cancer Biology Training Program 5T32CA09594. ",

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doi = "10.1016/S0014-5793(03)00558-1",

language = "English (US)",

volume = "546",

pages = "185--188",

journal = "FEBS Letters",

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number = "2-3",

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TY - JOUR

T1 - Actin dependent CD95 internalization is specific for Type I cells

AU - Algeciras-Schimnich, Alicia

AU - Peter, Marcus E.

N1 - Funding Information: We like to thank Dr. P. Krammer for providing us with anti-APO-1 and Dr. H. Walczak for his generous gift of LzCD95L. We would also like to thank E. Pietras for excellent technical assistance, S. Vijayan for performing the HS1 Western blot, and B. Barnhart for critically reading the manuscript. This work has been funded by the NIH Grant GM61712. A.A.-S. was supported by the Cancer Biology Training Program 5T32CA09594.

PY - 2003/7/10

Y1 - 2003/7/10

N2 - CD95 Type I and Type II cells differ in the requirement for mitochondrial contribution to the execution of apoptosis. Mitochondria are required to amplify the apoptosis signal only in Type II cells. Here we show that CD95 internalizes in an actin dependent manner only in Type I cells and that the two CD95 apoptosis cell types differ in their threshold of active caspase-8 required to execute apoptosis. The data suggest that CD95 is linked to the cytoskeleton in different ways in the two cell types and that they have adapted to different levels of active caspase-8 generated at the activated receptor.

AB - CD95 Type I and Type II cells differ in the requirement for mitochondrial contribution to the execution of apoptosis. Mitochondria are required to amplify the apoptosis signal only in Type II cells. Here we show that CD95 internalizes in an actin dependent manner only in Type I cells and that the two CD95 apoptosis cell types differ in their threshold of active caspase-8 required to execute apoptosis. The data suggest that CD95 is linked to the cytoskeleton in different ways in the two cell types and that they have adapted to different levels of active caspase-8 generated at the activated receptor.

KW - Actin

KW - Apoptosis

KW - Caspase-8

KW - Endocytosis

KW - Fas

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U2 - 10.1016/S0014-5793(03)00558-1

DO - 10.1016/S0014-5793(03)00558-1

M3 - Article

C2 - 12832037

AN - SCOPUS:0038198615

VL - 546

SP - 185

EP - 188

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -

Actin dependent CD95 internalization is specific for Type I cells

Abstract

Keywords

ASJC Scopus subject areas

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