Abstract
CD95 Type I and Type II cells differ in the requirement for mitochondrial contribution to the execution of apoptosis. Mitochondria are required to amplify the apoptosis signal only in Type II cells. Here we show that CD95 internalizes in an actin dependent manner only in Type I cells and that the two CD95 apoptosis cell types differ in their threshold of active caspase-8 required to execute apoptosis. The data suggest that CD95 is linked to the cytoskeleton in different ways in the two cell types and that they have adapted to different levels of active caspase-8 generated at the activated receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 185-188 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 546 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jul 10 2003 |
Funding
We like to thank Dr. P. Krammer for providing us with anti-APO-1 and Dr. H. Walczak for his generous gift of LzCD95L. We would also like to thank E. Pietras for excellent technical assistance, S. Vijayan for performing the HS1 Western blot, and B. Barnhart for critically reading the manuscript. This work has been funded by the NIH Grant GM61712. A.A.-S. was supported by the Cancer Biology Training Program 5T32CA09594.
Keywords
- Actin
- Apoptosis
- Caspase-8
- Endocytosis
- Fas
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology