Activity-induced manganese-dependent MRI (AIM-MRI) and functional MRI in awake rabbits during somatosensory stimulation

Matthew P. Schroeder, Craig Weiss, Daniele Procissi, Lei Wang, John F Disterhoft*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Activity-induced manganese-dependent MRI (AIM-MRI) is a powerful tool to track system-wide neural activity using high resolution, quantitative T1-weighted MRI in animal models and has significant advantages for investigating neural activity over other modalities including BOLD fMRI. With AIM-MRI, Mn2+ ions enter neurons via voltage-gated calcium channels preferentially active during the time of experimental exposure. A broad range of AIM-MRI studies using different species studying different phenomena have been performed, but few of these studies provide a systematic evaluation of the factors influencing the detection of Mn2+ such as dosage and the temporal characteristics of Mn2+ uptake.We identified an optimal dose of Mn2+ (25mg/kg, s.c.) in order to characterize the time-course of Mn2+ accumulation in active neural regions in the rabbit. T1-weighted MRI and functional MRI were collected 0-3, 6-9, and 24-27h post-Mn2+ injection while the vibrissae on the right side were vibrated. Significant BOLD activation in the left somatosensory (SS) cortex and left ventral posteromedial (VPM) thalamic nucleus was detected during whisker vibration. T1-weighted signal intensities were extracted from these regions, their corresponding contralateral regions and the visual cortex (to serve as controls). A significant elevation in T1-weighted signal intensity in the left SS cortex (relative to right) was evident 6-9 and 24-27h post-Mn2+ injection while the left VPM thalamus showed a significant enhancement (relative to the right) only during the 24-27h session. Visual cortex showed no hemispheric difference at any timepoint. Our results suggest that studies employing AIM-MRI would benefit by conducting experimental manipulations 6-24h after subcutaneous MnCl2 injections to optimize the concentration of contrast agent in the regions active during the exposure.

Original languageEnglish (US)
Pages (from-to)72-80
Number of pages9
JournalNeuroimage
Volume126
DOIs
StatePublished - Feb 1 2016

Fingerprint

Manganese
Magnetic Resonance Imaging
Rabbits
Vibrissae
Somatosensory Cortex
Visual Cortex
Ventral Thalamic Nuclei
Injections
Subcutaneous Injections
Calcium Channels
Vibration
Thalamus
Contrast Media
Animal Models
Ions
Neurons

Keywords

  • Activity-induced
  • Awake animal MRI
  • Functional magnetic resonance imaging
  • Manganese-enhanced MRI
  • Rabbit
  • Somatosensory

ASJC Scopus subject areas

  • Neurology
  • Cognitive Neuroscience

Cite this

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title = "Activity-induced manganese-dependent MRI (AIM-MRI) and functional MRI in awake rabbits during somatosensory stimulation",
abstract = "Activity-induced manganese-dependent MRI (AIM-MRI) is a powerful tool to track system-wide neural activity using high resolution, quantitative T1-weighted MRI in animal models and has significant advantages for investigating neural activity over other modalities including BOLD fMRI. With AIM-MRI, Mn2+ ions enter neurons via voltage-gated calcium channels preferentially active during the time of experimental exposure. A broad range of AIM-MRI studies using different species studying different phenomena have been performed, but few of these studies provide a systematic evaluation of the factors influencing the detection of Mn2+ such as dosage and the temporal characteristics of Mn2+ uptake.We identified an optimal dose of Mn2+ (25mg/kg, s.c.) in order to characterize the time-course of Mn2+ accumulation in active neural regions in the rabbit. T1-weighted MRI and functional MRI were collected 0-3, 6-9, and 24-27h post-Mn2+ injection while the vibrissae on the right side were vibrated. Significant BOLD activation in the left somatosensory (SS) cortex and left ventral posteromedial (VPM) thalamic nucleus was detected during whisker vibration. T1-weighted signal intensities were extracted from these regions, their corresponding contralateral regions and the visual cortex (to serve as controls). A significant elevation in T1-weighted signal intensity in the left SS cortex (relative to right) was evident 6-9 and 24-27h post-Mn2+ injection while the left VPM thalamus showed a significant enhancement (relative to the right) only during the 24-27h session. Visual cortex showed no hemispheric difference at any timepoint. Our results suggest that studies employing AIM-MRI would benefit by conducting experimental manipulations 6-24h after subcutaneous MnCl2 injections to optimize the concentration of contrast agent in the regions active during the exposure.",
keywords = "Activity-induced, Awake animal MRI, Functional magnetic resonance imaging, Manganese-enhanced MRI, Rabbit, Somatosensory",
author = "Schroeder, {Matthew P.} and Craig Weiss and Daniele Procissi and Lei Wang and Disterhoft, {John F}",
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T1 - Activity-induced manganese-dependent MRI (AIM-MRI) and functional MRI in awake rabbits during somatosensory stimulation

AU - Schroeder, Matthew P.

AU - Weiss, Craig

AU - Procissi, Daniele

AU - Wang, Lei

AU - Disterhoft, John F

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Activity-induced manganese-dependent MRI (AIM-MRI) is a powerful tool to track system-wide neural activity using high resolution, quantitative T1-weighted MRI in animal models and has significant advantages for investigating neural activity over other modalities including BOLD fMRI. With AIM-MRI, Mn2+ ions enter neurons via voltage-gated calcium channels preferentially active during the time of experimental exposure. A broad range of AIM-MRI studies using different species studying different phenomena have been performed, but few of these studies provide a systematic evaluation of the factors influencing the detection of Mn2+ such as dosage and the temporal characteristics of Mn2+ uptake.We identified an optimal dose of Mn2+ (25mg/kg, s.c.) in order to characterize the time-course of Mn2+ accumulation in active neural regions in the rabbit. T1-weighted MRI and functional MRI were collected 0-3, 6-9, and 24-27h post-Mn2+ injection while the vibrissae on the right side were vibrated. Significant BOLD activation in the left somatosensory (SS) cortex and left ventral posteromedial (VPM) thalamic nucleus was detected during whisker vibration. T1-weighted signal intensities were extracted from these regions, their corresponding contralateral regions and the visual cortex (to serve as controls). A significant elevation in T1-weighted signal intensity in the left SS cortex (relative to right) was evident 6-9 and 24-27h post-Mn2+ injection while the left VPM thalamus showed a significant enhancement (relative to the right) only during the 24-27h session. Visual cortex showed no hemispheric difference at any timepoint. Our results suggest that studies employing AIM-MRI would benefit by conducting experimental manipulations 6-24h after subcutaneous MnCl2 injections to optimize the concentration of contrast agent in the regions active during the exposure.

AB - Activity-induced manganese-dependent MRI (AIM-MRI) is a powerful tool to track system-wide neural activity using high resolution, quantitative T1-weighted MRI in animal models and has significant advantages for investigating neural activity over other modalities including BOLD fMRI. With AIM-MRI, Mn2+ ions enter neurons via voltage-gated calcium channels preferentially active during the time of experimental exposure. A broad range of AIM-MRI studies using different species studying different phenomena have been performed, but few of these studies provide a systematic evaluation of the factors influencing the detection of Mn2+ such as dosage and the temporal characteristics of Mn2+ uptake.We identified an optimal dose of Mn2+ (25mg/kg, s.c.) in order to characterize the time-course of Mn2+ accumulation in active neural regions in the rabbit. T1-weighted MRI and functional MRI were collected 0-3, 6-9, and 24-27h post-Mn2+ injection while the vibrissae on the right side were vibrated. Significant BOLD activation in the left somatosensory (SS) cortex and left ventral posteromedial (VPM) thalamic nucleus was detected during whisker vibration. T1-weighted signal intensities were extracted from these regions, their corresponding contralateral regions and the visual cortex (to serve as controls). A significant elevation in T1-weighted signal intensity in the left SS cortex (relative to right) was evident 6-9 and 24-27h post-Mn2+ injection while the left VPM thalamus showed a significant enhancement (relative to the right) only during the 24-27h session. Visual cortex showed no hemispheric difference at any timepoint. Our results suggest that studies employing AIM-MRI would benefit by conducting experimental manipulations 6-24h after subcutaneous MnCl2 injections to optimize the concentration of contrast agent in the regions active during the exposure.

KW - Activity-induced

KW - Awake animal MRI

KW - Functional magnetic resonance imaging

KW - Manganese-enhanced MRI

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KW - Somatosensory

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