Acute hormonal responses to the gonadotropin releasing hormone agonist leuprolide: Dose-response studies and comparison to nafarelin - A clinical research center study

Robert L. Rosenfield*, Nancy Perovic, David A. Ehrmann, Randall B. Barnes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The hormonal responses to single subcutaneous injection of the GnRH agonist nafarelin have been shown to have considerable potential as a diagnostic test in a number of settings. Since nafarelin injection is no longer produced, studies were conducted to determine the dosage of leuprolide that would yield equivalent responses. Nafarelin 100 μg stimulates LH and FSH for 24 h, releasing about 7-fold more gonadotropin than this dose of natural GnRH, which accounts for its ability to elicit gonadal steroid responses. Normal adult men and women were randomized to receive leuprolide doses of 0.1, 1.0, or 10 μg/kg; a study extension evaluated doses up to 20 μg/kg in men. The responses of LH, FSH, testosterone, and estradiol were monitored for 24 h, and the data were compared to those previously obtained on nafarelin. Leuprolide dose of 10 μg/kg yielded LH responses similar to 1- 1.5 μg/kg nafarelin. However, this leuprolide dose unexpectedly released less FSH than nafarelin. Nevertheless, the gonadotropin responses were sufficient to elicit equivalent or greater sex steroid responses to leuprolide. These studies also further delineated sex-specific differences in pituitary responsiveness to challenge with GnRH agonists: men had a significantly lower baseline FSH level, greater LH release within the first hour, and lesser secretion of LH and FSH over the 24-h period. These studies indicate that leuprolide in a dosage of 10 μg/kg would be expected to be efficacious in testing the pituitary-gonadal axis in men and women.

Original languageEnglish (US)
Pages (from-to)3408-3411
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume81
Issue number9
DOIs
StatePublished - Sep 18 1996

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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