Adenosine caused a 2.5-fold increase in the sodium-dependent uptake of 2-aminoisobutyric acid in rat hepatocytes in primary culture following incubation for 3h. The range of stimulating concentrations of adenosine corresponded to that of cAMP formation. Adenosine increased the Vmax of the transport without altering the Km for 2-aminoisobutyric acid. These effects of adenosine were abolished by actinomycin D. N6-L-Phenylisopropyladenosine also caused twofold increase in the amino acid transport. These findings suggest that adenosine induces System A amino acid transport in a transcription-dependent manner, and that P,-purinergic receptors are mainly involved in this action of the nucleoside.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Biochemistry|
|State||Published - Jan 1 1991|
ASJC Scopus subject areas
- Molecular Biology