Adenovirus vector vaccination impacts NK cell Rrheostat function following lymphocytic choriomeningitis virus infection

Eryn Blass, Malika Aid, Amanda J. Martinot, Rafael A. Larocca, Zi Han Kang, Alexander Badamchi-Zadeh, Pablo Penaloza-MacMaster, R. Keith Reeves, Dan H. Barouch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Natural killer (NK) cells respond rapidly as a first line of defense against infectious pathogens. In addition, NK cells may provide a "rheostat" function and have been shown to reduce the magnitude of antigen-specific T cell responses following infection to avoid immunopathology. However, it remains unknown whether NK cells similarly modulate vaccine-elicited T cell responses following virus challenge. We used the lymphocytic choriomeningitis virus (LCMV) clone 13 infection model to address whether NK cells regulate T cell responses in adenovirus vectorvaccinated mice following challenge. As expected, NK cell depletion in unvaccinated mice resulted in increased virus-specific CD4+ and CD8+ T cell responses and immunopathology following LCMV challenge. In contrast, NK cell depletion had minimal to no impact on antigen-specific T cell responses in mice that were vaccinated with an adenovirus serotype 5 (Ad5)-GP vector prior to LCMV challenge. Moreover, NK cell depletion in vaccinated mice prior to challenge did not result in immunopathology and did not compromise protective efficacy. These data suggest that adenovirus vaccine-elicited T cells may be less sensitive to NK cell rheostat regulation than T cells primed by LCMV infection.

Original languageEnglish (US)
Article numbere02103-17
JournalJournal of virology
Volume92
Issue number11
DOIs
StatePublished - Jun 1 2018

Keywords

  • Adenoviruses
  • Lymphocytic choriomeningitis virus
  • NK cells
  • Vaccines

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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