Adherence of human basophils to cultured umbilical vein endothelial cells

B. S. Bochner, P. T. Peachell, K. E. Brown, R. P. Schleimer

Research output: Contribution to journalArticlepeer-review

71 Scopus citations


The mechanism by which circulating human basophils adhere to vascular endothelium and migrate to sites of allergic reactions is unknown. Agents have been identified which stimulate the adherence of purified basophils to cultured human umbilical vein vascular endothelial cells (HuVEC). Treatment of HuVEC with interleukin 1, tumor necrosis factor (TNF), bacterial endotoxin, and 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in time and dose-dependent increases of adhesiveness for basophils. Coincubation of basophils and HuVEC for 10 min with C5a, formyl-methionyl-leucyl-phenylalanine, the calcium ionophore A23187, platelet-activating factor, TNF, and TPA also resulted in significant dose-dependent increases in basophil adherence; this effect resulted from activation of the basophil. Adherence of basophils to HuVEC was time and temperature dependent, required divalent cations, and was unaffected by glucocorticoids. Monoclonal antibody 60.3, directed against the β-subunit of the leukocyte adherence complex CD18, inhibited the binding of basophils to HuVEC. Adherence of basophils to vascular endothelium may be important in initiating basophil infiltrates in vivo.

Original languageEnglish (US)
Pages (from-to)1355-1364
Number of pages10
JournalJournal of Clinical Investigation
Issue number5
StatePublished - 1988

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Adherence of human basophils to cultured umbilical vein endothelial cells'. Together they form a unique fingerprint.

Cite this