Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

Gong Bo Li, Guang Xiu Lu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10. days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as " non-dendritic cells" or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these " junk cells" are actually qualified dendritic cells.

Original languageEnglish (US)
Pages (from-to)4-6
Number of pages3
JournalCellular Immunology
Volume264
Issue number1
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Adherent cell
  • Bone marrow
  • Dendritic cell

ASJC Scopus subject areas

  • Immunology

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