Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

Gong Bo Li; Guang Xiu Lu

doi:10.1016/j.cellimm.2010.05.009

Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

Gong Bo Li, Guang Xiu Lu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10. days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as " non-dendritic cells" or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these " junk cells" are actually qualified dendritic cells.

Original language	English (US)
Pages (from-to)	4-6
Number of pages	3
Journal	Cellular Immunology
Volume	264
Issue number	1
DOIs	https://doi.org/10.1016/j.cellimm.2010.05.009
State	Published - 2010
Externally published	Yes

Keywords

Adherent cell
Bone marrow
Dendritic cell

ASJC Scopus subject areas

Immunology

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10.1016/j.cellimm.2010.05.009

Cite this

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title = "Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells",

abstract = "A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10. days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as {"} non-dendritic cells{"} or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these {"} junk cells{"} are actually qualified dendritic cells.",

keywords = "Adherent cell, Bone marrow, Dendritic cell",

author = "Li, {Gong Bo} and Lu, {Guang Xiu}",

note = "Funding Information: This research was supported by The Hi-Tech Research and Development of China (863 program) Grant No. 2006AA02A102 ; the National Basic Research Program of China (973 program) Grant No. 2007CB947900 .",

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doi = "10.1016/j.cellimm.2010.05.009",

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T1 - Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

AU - Li, Gong Bo

AU - Lu, Guang Xiu

N1 - Funding Information: This research was supported by The Hi-Tech Research and Development of China (863 program) Grant No. 2006AA02A102 ; the National Basic Research Program of China (973 program) Grant No. 2007CB947900 .

PY - 2010

Y1 - 2010

N2 - A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10. days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as " non-dendritic cells" or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these " junk cells" are actually qualified dendritic cells.

AB - A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10. days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as " non-dendritic cells" or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these " junk cells" are actually qualified dendritic cells.

KW - Adherent cell

KW - Bone marrow

KW - Dendritic cell

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Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

Abstract

Keywords

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