Adhesion of DOPA-functionalized model membranes to hard and soft surfaces

Murat Guvendiren, David Brass, Phillip B. Messersmith, Kenneth R. Shull*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual amino acid, 3,4-dihydroxy-L-phenylalanine (DOPA), which is largely responsible for their cohesive and adhesive strengths. In this study, we incorporated DOPA into diblock and triblock polymers and developed a membrane contact experiment to assess the adhesive interactions of these materials with TiO2 and tissue surfaces. In a typical experiment a micrometer-thick DOPA-functionalized elastomeric membrane is attached to the end of a cylindrical glass tube. Application of a positive pressure to the tube brings the membrane into contact with the surface of interest. The negative pressure needed to separate the membrane from the substrate is a measure of the strength of the adhesive interaction. The test confirms previous results obtained with TiO2 substrates. Because the membrane geometry is well suited for rough or chemically heterogeneous surfaces, it is ideal for studies of tissue adhesion. DOPA was found to give strong adhesion to tissue surfaces, with the strongest adhesion obtained when the DOPA groups were oxidized while in contact with the tissue surface.

Original languageEnglish (US)
Pages (from-to)631-645
Number of pages15
JournalJournal of Adhesion
Volume85
Issue number9
DOIs
StatePublished - Sep 21 2009

Keywords

  • Block Copolymers
  • DOPA
  • Marine Mussel Mimetics
  • Membrane
  • Tissue Adhesion

ASJC Scopus subject areas

  • Chemistry(all)
  • Mechanics of Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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