Adipose-derived stromal cells and mineralized extracellular matrix delivery by a human decellularized amniotic membrane in periodontal tissue engineering

Dilcele Silva Moreira Dziedzic, Bassam Felipe Mogharbel, Ana Carolina Irioda, Priscila Elias Ferreira Stricker, Maiara Carolina Perussolo, Célia Regina Cavichiolo Franco, Hsueh Wen Chang, Eltyeb Abdelwahid, Katherine Athayde Teixeira de Carvalho*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

Original languageEnglish (US)
Article number606
JournalMembranes
Volume11
Issue number8
DOIs
StatePublished - Aug 2021

Keywords

  • Adipose-derived stromal cells
  • Bone tissue engineering
  • Human amniotic membrane
  • Periodontal regeneration
  • Stem cell transplantation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Process Chemistry and Technology
  • Filtration and Separation

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