Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM)

George E. Cutsail, Joshua Telser, Brian M. Hoffman*

*Corresponding author for this work

Research output: Contribution to journalReview article

18 Scopus citations

Abstract

The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.

Original languageEnglish (US)
Pages (from-to)1370-1394
Number of pages25
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1853
Issue number6
DOIs
StatePublished - Jun 1 2015

Keywords

  • Advanced paramagnetic resonance spectroscopy of iron-sulfur proteins
  • Electron nuclear double resonance (ENDOR)
  • Electron spin echo envelope modulation (ESEEM)

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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