TY - JOUR
T1 - Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins
T2 - Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM)
AU - Cutsail, George E.
AU - Telser, Joshua
AU - Hoffman, Brian M.
N1 - Funding Information:
The authors thank Jacques Meyer for helpful conversations and careful reading of this manuscript. The authors also thank Jacques Meyer for providing the A. aeolicus Fd1 sample, Jose Moura, UNL, Lisbon, Portugal for the sample of Desulfovibrio gigas Fd, and Mary Claire Kennedy, Medical College of Wisconsin, for the sample of Ectothiorhodospira halophila HiPIP. This work was supported by the National Institutes of Health ( GM111097 to B.M.H.) and the National Science Foundation ( MCB-1118613 to B.M.H., and DGE-0824162 to G.E.C.).
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
AB - The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
KW - Advanced paramagnetic resonance spectroscopy of iron-sulfur proteins
KW - Electron nuclear double resonance (ENDOR)
KW - Electron spin echo envelope modulation (ESEEM)
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U2 - 10.1016/j.bbamcr.2015.01.025
DO - 10.1016/j.bbamcr.2015.01.025
M3 - Review article
C2 - 25686535
AN - SCOPUS:84939966109
SN - 0167-4889
VL - 1853
SP - 1370
EP - 1394
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 6
ER -