Advances in localized surface plasmon resonance spectroscopy biosensing

Laura B. Sagle, Laura K. Ruvuna, Julia A. Ruemmele, Richard P. Van Duyne*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

123 Scopus citations


In recent years, localized surface plasmon resonance (LSPR) spectroscopy advancements have made it a sensitive, flexible tool for probing biological interactions. Here, we describe the basic principles of this nanoparticle-based sensing technique, the ways nanoparticles can be tailored to optimize sensing, and examples of novel LSPR spectroscopy applications. These include detecting small molecules via protein conformational changes and resonance LSPR spectroscopy, as well as coupling LSPR with mass spectrometry to identify bound analytes. The last few sections highlight the advantages of single nanoparticle LSPR, in that it lowers limits of detection, allows multiplexing on the nanometer scale, and enables free diffusion of sensors in solution. The cases discussed herein illustrate creative ways that LSPR spectroscopy has been improved to achieve new sensing capabilities.

Original languageEnglish (US)
Pages (from-to)1447-1462
Number of pages16
Issue number8
StatePublished - Oct 2011


  • LSPR
  • biosensing
  • imaging
  • multiplex
  • nanoparticle
  • tracking

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)


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