Affinity chromatography purification of a cyclic nucleotide phosphodiesterase using immobilized modulator protein, a troponin C-like protein from brain

D. Martin Watterson*, Thomas C. Vanaman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Modulator protein, a brain troponin C-like protein, has been coupled to Sepharose 4B using conditions that allow retention of phosphodiesterase stimulatory activity. This conjugate has been used to directly demonstrate the calcium dependent formation of a reversible modulator protein-phosphodiesterase complex and to purify a cyclic nucleotide phosphodiesterase by affinity chromatography.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume73
Issue number1
DOIs
StatePublished - Nov 8 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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