Modulator protein, a brain troponin C-like protein, has been coupled to Sepharose 4B using conditions that allow retention of phosphodiesterase stimulatory activity. This conjugate has been used to directly demonstrate the calcium dependent formation of a reversible modulator protein-phosphodiesterase complex and to purify a cyclic nucleotide phosphodiesterase by affinity chromatography.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Nov 8 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology