TY - JOUR
T1 - Affinity chromatography purification of a cyclic nucleotide phosphodiesterase using immobilized modulator protein, a troponin C-like protein from brain
AU - Watterson, D. Martin
AU - Vanaman, Thomas C.
PY - 1976/11/8
Y1 - 1976/11/8
N2 - Modulator protein, a brain troponin C-like protein, has been coupled to Sepharose 4B using conditions that allow retention of phosphodiesterase stimulatory activity. This conjugate has been used to directly demonstrate the calcium dependent formation of a reversible modulator protein-phosphodiesterase complex and to purify a cyclic nucleotide phosphodiesterase by affinity chromatography.
AB - Modulator protein, a brain troponin C-like protein, has been coupled to Sepharose 4B using conditions that allow retention of phosphodiesterase stimulatory activity. This conjugate has been used to directly demonstrate the calcium dependent formation of a reversible modulator protein-phosphodiesterase complex and to purify a cyclic nucleotide phosphodiesterase by affinity chromatography.
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U2 - 10.1016/0006-291X(76)90494-0
DO - 10.1016/0006-291X(76)90494-0
M3 - Article
C2 - 187190
AN - SCOPUS:0017190359
SN - 0006-291X
VL - 73
SP - 40
EP - 46
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -