Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg

Jessica E. Hornick; Francesca E. Duncan; Mingxuan Sun; Ryo Kawamura; John F. Marko; Teresa K. Woodruff

doi:10.1007/s10815-015-0453-y

Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg

Jessica E. Hornick, Francesca E. Duncan, Mingxuan Sun, Ryo Kawamura, John F. Marko, Teresa K. Woodruff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Purpose: The incidence of aneuploidy in eggs from women of advanced reproductive age can exceed 60 %, making the mammalian egg a unique model system to study the mechanisms of chromosome segregation errors. Methods: Here we applied a novel biophysical chromosome stretching approach to quantify mechanical stiffness of meiotic chromosomes in the mammalian egg and then documented how these properties changed in a mouse model of physiologic reproductive aging. Results: We found significant differences in chromosome micromechanics, and thus in higher order chromosome structure, coincident with advanced reproductive age, a time that is also unequivocally associated with an increase in egg aneuploidy. Conclusions: These findings have important implications for both reproductive and cancer biology where aneuploidy plays a central role in aging related disease states.

Original language	English (US)
Pages (from-to)	765-769
Number of pages	5
Journal	Journal of Assisted Reproduction and Genetics
Volume	32
Issue number	5
DOIs	https://doi.org/10.1007/s10815-015-0453-y
State	Published - May 1 2015

Funding

This work was supported by grants from the National Institutes of Health (U54HD041857 to TKW and U54HD076188, U54CA143869 and R01GM105847 to JFM) and the National Science Foundation (NSF) (MCB-1022117 and DMR-1206868 to JFM).

Keywords

Chromosome
Mammalian
Meiosis
Micromechanics
Reproductive aging

ASJC Scopus subject areas

Reproductive Medicine
Genetics
Obstetrics and Gynecology
Developmental Biology
Genetics(clinical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10815-015-0453-y

Cite this

@article{184429a3a0fa4ea8b5fc599000c727f8,

title = "Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg",

abstract = "Purpose: The incidence of aneuploidy in eggs from women of advanced reproductive age can exceed 60 %, making the mammalian egg a unique model system to study the mechanisms of chromosome segregation errors. Methods: Here we applied a novel biophysical chromosome stretching approach to quantify mechanical stiffness of meiotic chromosomes in the mammalian egg and then documented how these properties changed in a mouse model of physiologic reproductive aging. Results: We found significant differences in chromosome micromechanics, and thus in higher order chromosome structure, coincident with advanced reproductive age, a time that is also unequivocally associated with an increase in egg aneuploidy. Conclusions: These findings have important implications for both reproductive and cancer biology where aneuploidy plays a central role in aging related disease states.",

keywords = "Chromosome, Mammalian, Meiosis, Micromechanics, Reproductive aging",

author = "Hornick, {Jessica E.} and Duncan, {Francesca E.} and Mingxuan Sun and Ryo Kawamura and Marko, {John F.} and Woodruff, {Teresa K.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (U54HD041857 to TKW and U54HD076188, U54CA143869 and R01GM105847 to JFM) and the National Science Foundation (NSF) (MCB-1022117 and DMR-1206868 to JFM). Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media New York.",

year = "2015",

month = may,

day = "1",

doi = "10.1007/s10815-015-0453-y",

language = "English (US)",

volume = "32",

pages = "765--769",

journal = "Journal of Assisted Reproduction and Genetics",

issn = "1058-0468",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg

AU - Hornick, Jessica E.

AU - Duncan, Francesca E.

AU - Sun, Mingxuan

AU - Kawamura, Ryo

AU - Marko, John F.

AU - Woodruff, Teresa K.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (U54HD041857 to TKW and U54HD076188, U54CA143869 and R01GM105847 to JFM) and the National Science Foundation (NSF) (MCB-1022117 and DMR-1206868 to JFM). Publisher Copyright: © 2015, Springer Science+Business Media New York.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Purpose: The incidence of aneuploidy in eggs from women of advanced reproductive age can exceed 60 %, making the mammalian egg a unique model system to study the mechanisms of chromosome segregation errors. Methods: Here we applied a novel biophysical chromosome stretching approach to quantify mechanical stiffness of meiotic chromosomes in the mammalian egg and then documented how these properties changed in a mouse model of physiologic reproductive aging. Results: We found significant differences in chromosome micromechanics, and thus in higher order chromosome structure, coincident with advanced reproductive age, a time that is also unequivocally associated with an increase in egg aneuploidy. Conclusions: These findings have important implications for both reproductive and cancer biology where aneuploidy plays a central role in aging related disease states.

AB - Purpose: The incidence of aneuploidy in eggs from women of advanced reproductive age can exceed 60 %, making the mammalian egg a unique model system to study the mechanisms of chromosome segregation errors. Methods: Here we applied a novel biophysical chromosome stretching approach to quantify mechanical stiffness of meiotic chromosomes in the mammalian egg and then documented how these properties changed in a mouse model of physiologic reproductive aging. Results: We found significant differences in chromosome micromechanics, and thus in higher order chromosome structure, coincident with advanced reproductive age, a time that is also unequivocally associated with an increase in egg aneuploidy. Conclusions: These findings have important implications for both reproductive and cancer biology where aneuploidy plays a central role in aging related disease states.

KW - Chromosome

KW - Mammalian

KW - Meiosis

KW - Micromechanics

KW - Reproductive aging

UR - http://www.scopus.com/inward/record.url?scp=84939980615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939980615&partnerID=8YFLogxK

U2 - 10.1007/s10815-015-0453-y

DO - 10.1007/s10815-015-0453-y

M3 - Article

C2 - 25758987

AN - SCOPUS:84939980615

SN - 1058-0468

VL - 32

SP - 765

EP - 769

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 5

ER -

Age-associated alterations in the micromechanical properties of chromosomes in the mammalian egg

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this