Age-related changes in the cerebrospinal fluid outflow glycosaminoglycans

Paul A. Knepper*, Ralph K. Losey, Jennifer A. Collins, David G. McLone, Hyman G. Weinstein, Moira Breen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The glycosaminoglycan distribution patterns of the cerebrospinal fluid (CSF) outflow pathway, dura mater and cerebral cortex of young New Zealand red rabbits and 1-, 3- and 12-week-old C-57 mice were identified by analyses of the glycosaminoglycan moieties and by the use of zone electrophoresis. The glycosaminoglycans were identified by specific degradation procedures, i.e., hyaluronate lyase, chondroitin ABC lyase, endo-gb-D-galactosidase and nitrous acid treatment. The CSF outflow pathway and dura mater glycosaminoglycan components were primarily hyaluronic acid and chondroitin sulfatedermatan sulfate, whereas the cerebral cortex glycosaminoglycan components were hyaluronic acid, chondroitin sulfatedermatan sulfate, keratan sulfate and heparan sulfate. The glycosaminoglycan components of the dura mater and cerebral cortex decreased and those of the CSF outflow pathway increased as a function of age. These results demonstrate the feasibility of analyses of the CSF outflow pathway glycosaminoglycan components and suggest that topographical changes in the glycosaminoglycan distribution profiles may contribute to the pattern of cerebrospinal fluid outflow.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalNeurobiology of Aging
Issue number2
StatePublished - 1983


  • Age-related changes
  • Cerebrospinal fluid outflow pathway
  • Glycosaminoglycans

ASJC Scopus subject areas

  • General Neuroscience
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology


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