Age-related differences in the metabolism of proteoglycans in bovine articular cartilage explants maintained in the presence of insulin-like growth factor i

Judith Barone-Varelas, Thomas J. Schnitzer, Qi Meng, Lori Otten, Eugene J M A Thonar

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Articular cartilage explants from bovine calf and steer were cultured for up to 19 days in medium with or without 20% fetal bovine serum (FBS) or insulin-like growth factor I (IGF-I). Lower concentrations of IGF-I were required for maximal stimulation of PG synthesis in calf than in steer. In calf, but not in steer, IGF-I was as effective as 20% FBS in stimulating PG synthesis. The stimulation by IGF-I or FBS was not accompanied at either age by alterations in the composition of the aggregating PGs nor by changes in the proportions of CS-rich and CS-poor PG subpopulations. In calf, IGF-I and FBS did not markedly alter the rate of turnover of either the 35S-PGs synthesized in vitro or the unlabeled PGs. In steer, explants cultured in the absence of IGF-I or FBS exhibited very fast rates of turnover and depletion of matrix PG with time; IGF-I and FBS were both effective in reducing the turnover rate of 35S-PGs and unlabeled PGs and in preventing PG depletion.

Original languageEnglish (US)
Pages (from-to)101-120
Number of pages20
JournalConnective tissue research
Volume26
Issue number1-2
DOIs
StatePublished - 1991

Keywords

  • Age-related
  • Cartilage
  • Insulin-like growth factor I
  • Proteoglycan synthesis
  • Proteoglycan turnover

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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