Age-related effect of cells-donors of nuclei on efficiency of development of cloned rabbit embryos

I. S. Lagutina*, M. N. Mezina, M. I. Prokof'ev, V. Ya Chernykh, V. V. Galat

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We studied the capacity of nuclei of rabbit fibroblasts taken from various developmental stages for reprogramming in the cytoplasm of mature aging enucleated oocytes and development of the cloned embryos to the preimplantation stages. A negative correlation was found between the age of an animal-donor of fibroblasts and efficiency of the development of cloned embryos (rmorula-blastocyst = -0.826, rblastocyst = -0.7139). A reliably decreased capacity for reprogramming of the nuclei of donor fibroblasts was shown upon transition from prenatal development to the postnatal one, as well as a trend to a decreased capacity of nuclei for reprogramming during aging. Aging of cells in the culture, at least until the 10th passage, did not affect the capacity of the nuclei of fetal fibroblasts for reprogramming and development of cloned embryos.

Original languageEnglish (US)
Pages (from-to)130-139
Number of pages10
JournalOntogenez
Volume32
Issue number2
StatePublished - Dec 1 2001

Keywords

  • Fibroblasts
  • Nuclear reprogramming.
  • Nuclear transfer
  • Oocytes
  • Rabbit
  • Reconstructed embryos

ASJC Scopus subject areas

  • Medicine(all)

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