Agonist-induced regulation of the neuronal nicotinic acetylcholine receptor pf PC12 cells

D. Robinson, R. McGee

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Pheochromocytoma cells, clone PC12, possess neuronal nicotinic acetylcholine (ACh) receptors which can be activated by an agonist to produce a transient transmembrane cation flux similar to that seen with neuromuscular nicotinic ACh receptors. We have observed that these neuronal nicotinic ACh receptors are subject to long term regulation by the cholinergic agonist carbamoylcholine (carbachol). Receptor function was measured by agonist-induced uptake of 86Rb+ into the cells. Chronic exposure to concentrations of carbachol that caused receptor activation induced an adaptation (down-regulation) of the receptors, seen as a decrease in the responsiveness of the cells to a subsequent exposure to carbachol. The extent of the decreased responsiveness was proportional to the concentration of carbachol between 50 μM and 10 mM and was distinct from acute receptor desensitization. The concentrations of carbachol that caused down-regulation were greater than those that caused maximum receptor activation, but were similar to those that greatly enhanced desensitization. This suggested that promotion of the desensitized state of the receptor could be true stimulus for down-regulation. Adaptation was observed initially after several hours of exposure and maximally within about 7 days. Recovery from the down-regulated state required several days of growth in the absence of carbachol. Treatment with the antagonist mecamylamine did not cause a similar change in the responsiveness of the cells. However, concurrent exposure to carbachol and mecamylamine prevented down-regulation. Therefore, it appeared that receptor activation was necessary for regulation to occur. Comparison of the cellular responses of chronically treated and control cells to acute stimulation with carbachol supported the hypothesis that the mechanism for this down-regulation in PC12 cells was a decrease in the number of ACh receptors.

Original languageEnglish (US)
Pages (from-to)409-417
Number of pages9
JournalMolecular Pharmacology
Volume27
Issue number4
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Pharmacology

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