Airway contractility in the precision-cut lung slice after cryopreservation

Sonia R. Rosner, Sumati Ram-Mohan, Jesus R. Paez-Cortez, Tera L. Lavoie, Maria L. Dowell, Lei Yuan, Xingbin Ai, Alan Fine, William C. Aird, Julian Solway, Jeffrey J. Fredberg, Ramaswamy Krishnan*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozenthawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.

Original languageEnglish (US)
Pages (from-to)876-881
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume50
Issue number5
DOIs
StatePublished - May 2014
Externally publishedYes

Keywords

  • Airway contractility
  • Airway smooth muscle
  • Cryopreservation
  • Precision-cut lung slice

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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  • Cite this

    Rosner, S. R., Ram-Mohan, S., Paez-Cortez, J. R., Lavoie, T. L., Dowell, M. L., Yuan, L., Ai, X., Fine, A., Aird, W. C., Solway, J., Fredberg, J. J., & Krishnan, R. (2014). Airway contractility in the precision-cut lung slice after cryopreservation. American journal of respiratory cell and molecular biology, 50(5), 876-881. https://doi.org/10.1165/rcmb.2013-0166MA