Akt isoforms differentially regulate neutrophil functions

Jia Chen, Haiyang Tang, Nissim Hay, Jingsong Xu, Richard D. Ye

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

In neutrophils, the phosphoinositide 3-kinase/Akt signaling cascade is involved in migration, degranulation, and O2- production. However, it is unclear whether the Akt kinase isoforms have distinct functions in neutrophil activation. Here we report functional differences between the 2 major Akt isoforms in neutrophil activation on the basis of studies in which we used individual Akt1 and Akt2 knockout mice. Akt2-/- neutrophils exhibited decreased cell migration, granule enzyme release, and O 2- production compared with wild-type and Akt1 -/- neutrophils. Surprisingly, Akt2 deficiency and pharmacologic inhibition of Akt also abrogated phorbol ester-induced O2- production, which was unaffected by treatment with the phosphoinositide 3-kinase inhibitor LY294002. The decreased O2- production in Akt2-/- neutrophils was accompanied by reduced p47phox phosphorylation and its membrane translocation, suggesting that Akt2 is important for the assembly of phagocyte nicotinamide adenine dinucleotide phosphate oxidase. In wild-type neutrophils, Akt2 but not Akt1 translocated to plasma membrane upon chemoattractant stimulation and to the leading edge in polarized neutrophils. In the absence of Akt2, chemoattractant-induced Akt protein phosphorylation was significantly reduced. These results demonstrate a predominant role of Akt2 in regulating neutrophil functions and provide evidence for differential activation of the 2 Akt isoforms in neutrophils.

Original languageEnglish (US)
Pages (from-to)4237-4246
Number of pages10
JournalBlood
Volume115
Issue number21
DOIs
StatePublished - May 27 2010

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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