Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro

Roman M. Natoli, Henry Yu, Megan Conti Mica Meislin, Pegah Abbasnia, Philip Roper, Aleksandra Vuchkovska, Xianghui Xiao, Stuart R. Stock, John J. Callaci*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: Alcohol consumption is a risk factor for impaired fracture healing, though the mechanism(s) by which this occurs are not well understood. Our laboratory has previously shown that episodic alcohol exposure of rodents negatively affects fracture callus development, callus biomechanics, and cellular signaling which regulates stem cell differentiation. Here, we examine whether alcohol alters chemokine expression and/or signaling activity in the mouse fracture callus during early fracture healing. Methods: A mouse model for alcohol-impaired tibia fracture healing was utilized. Early fracture callus was examined for alcohol-effects on tissue composition, expression of chemokines involved in MSC migration to the fracture site, and biomechanics. The effects of alcohol on MSC migration and cell adhesion receptors were examined in an in vitro system. Results: Mice exposed to alcohol showed decreased evidence of external callus formation, decreased callus-related osteopontin (OPN) expression levels, and decreased biomechanical stiffness. Alcohol exposure decreased rOPN-mediated MSC migration and integrin β1 receptor expression in vitro. Conclusions: The effects of alcohol exposure demonstrated here on fracture callus-associated OPN expression, rOPN-mediated MSC migration in vitro, and MSC integrin β1 receptor expression in vitro have not been previously reported. Understanding the effects of alcohol exposure on the early stages of fracture repair may allow timely initiation of treatment to mitigate the long-term complications of delayed healing and/or fracture non-union.

Original languageEnglish (US)
Article number101
JournalJournal of Orthopaedic Surgery and Research
Volume13
Issue number1
DOIs
StatePublished - Apr 27 2018

    Fingerprint

Keywords

  • Alcohol
  • Bone fracture
  • Fracture non-union
  • Integrin
  • Mesenchymal stem cell migration
  • Osteopontin

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this